黄瓜幼苗的美拉因介导的低温耐受性需要Ca2+/CPKs信号通路
Cheng Ma1, Zi-Qi Pei1, Qiao Zhu1
1College of Life Sciences, Northwest Normal University, Lanzhou, 730070, China.
Plant physiology and biochemistry : PPB
|July 27, 2024
概括
黑色素预处理通过增强信号和抗氧化防御来提高黄瓜幼苗对低温压力的耐受性. 这涉及Ca2+/依赖的蛋白激酶通路,对于Mel介导的冷硬性至关重要.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 黑素 (Mel) 是一种关键的植物生长调节剂,在应激反应中具有已知的作用.
- 低温 (LT) 压力显著抑制植物生长,需要采取提高作物弹性的策略.
- 了解植物对非生物压力的反应背后的分子机制对于农业的进步至关重要.
研究的目的:
- 调查Mel对黄瓜幼苗中LT诱导的生长抑制的缓解作用.
- 探索Ca2+/依赖的蛋白激酶 (CPKs) 信号通路在Mel介导的LT耐受性中的作用.
主要方法:
- 在暴露于LT压力 (10°C/6°C) 之前,黄瓜苗木先用Mel (100μM) 进行预处理.
- 测量包括Ca2+含量,CPK含量,基因表达 (Ca2+载体,CNGC,CPK,ICE-CBF-COR,MAPK),抗氧化酶活性和光合作用效率.
- 使用信号阻断剂 (LaCl3,EGTA) 和CPKs抑制剂 (TFP,W-7) 评估了Ca2+/CPKs通路的参与.
主要成果:
- 在LT压力下,Mel预处理显著增加了Ca2+和CPK水平,并上调相关基因表达.
- 梅尔增强了抗氧化剂防御和光合作用,同时提高了ICE-CBF-COR和MAPK通路基因的调节.
- 抑制Ca2+信号传递或CPKs活动减少了Mel的有益作用,证实了途径的参与.
结论:
- 黑色素有效地减轻了黄瓜幼苗中LT诱导的生长抑制.
- 在黄瓜中,Ca2+/CPKs信号通路对于Mel介导的LT耐受性增强至关重要.
- 结果提供了关于Mel在农业中的应用及其应激耐受机制的见解.
相关概念视频
Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
C4 Pathway and CAM
45.4K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.4K
Biological Clocks and Seasonal Responses
34.6K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.6K
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Adaptations that Reduce Water Loss
25.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.4K


