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结肠癌中长非编码RNA的差异表达:从转录基因分析的见解

Hadis Ahmadirad¹, Hossein Pourghadamyari², Morteza Hadizadeh³

¹Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Pathology, research and practice

|July 27, 2024

概括

结肠癌 (CC) 的发病率正在上升. 这项研究确定了两个长非编码RNAs (lncRNAs),FENDRR和LINC00092,在CC中表达率下降. 促剂高甲基化可能会导致这种情况,这表明lncRNAs是潜在的CC生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

在全球范围内,结肠癌 (CC) 的发病率正在增加.
长非编码RNAs (lncRNAs) 在基因调节和癌症发展中起着至关重要的作用,包括CC.

研究的目的:

调查特定 lncRNAs 在结肠癌发展中的功能.
为了识别潜在的 lncRNA 生物标志物用于 CC 诊断和治疗.

主要方法:

对CC组织中差异表达的lncRNAs (DElncRNAs) 和mRNAs (DEmRNAs) 的GEO数据集 (GSE104836) 的分析.
通过在31个结对的瘤和相邻的正常组织中评估表达水平,选择和验证FENDRR和LINC00092.
使用GEPIA2的生存分析和通过ROC方法的诊断能力评估.
使用甲基化特异性PCR (MSP) 调查促进体甲基化模式.

主要成果:

在所有阶段的结肠癌组织中,FENDRR和LINC00092的表达显著下降.
减少FENDRR表达与CC患者的总生存率下降相关.
这两种 lncRNAs 显示了 CC 和正常组织之间的显著区分能力.
与正常组织相比,在瘤组织中观察到FENDRR和LINC00092的促进子区域的高甲基化.

结论:

FENDRR和LINC00092作为结肠癌的潜在分子诊断生物标志物.
鉴定到的lncRNAs代表了CC治疗的有前途的治疗点.
促进物甲基化是调节结肠癌中FENDRR和LINC00092表达的关键机制.

关键词:

大肠癌是什么意思大肠癌是什么意思不同表达的 lncRNA.长长的非编码RNAs.促进剂甲基化促进剂甲基化

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