相关实验视频

Updated: Jun 18, 2025

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

循环单链核酸应用方面的进展

Xin-Yang Liu¹, Jian-Fei Tong¹, Ming-Yang Li¹

¹Department of Student team, College of Basic Medical Sciences, Naval Medical University, 200433, People's Republic of China.

Journal of biotechnology

|July 27, 2024

概括

循环核酸,包括循环RNA (circRNA) 和循环单链DNA (cssDNA),提供了增强的稳定性. 本综述探讨了它们多样化的合成方法和在基因表达和生物技术中的重要应用.

科学领域:

生物化学生物化学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

循环核酸具有固有的生物稳定性,因为它们缺乏5'和3'末端,使它们能够抵抗外核酶降解.
研究主要集中在循环单链核酸,特别是循环RNA (circRNA) 和循环单链DNA (cssDNA).

研究的目的:

综合审查各种类型的循环单链核酸.
详细介绍 circRNA 和 cssDNA 所使用的多种合成方法.
突出这些循环核酸的当前和潜在应用.

主要方法:

循环RNA (circRNA) 合成涉及诸如拉索驱动循环,内配对循环和人工合成等方法.
循环单链DNA (cssDNA) 制备利用化学结合,模板导向酶结合和基于CircLigase的技术.
该综述综合了关于循环核酸研究现有文献中的信息.

主要成果:

circRNA在基因表达调节和各种疾病的治疗干预中发挥着至关重要的作用.
cssDNA在滚动圆放大 (RCA) 技术和aptamers和第二信使的生物保护中起着重要作用.
循环核酸的独特结构特征有助于其稳定性和多样化的功能.

结论:

循环单链核酸是具有重要的生物学作用的稳定和多功能生物分子类别.
了解它们的合成和应用为未来分子生物学和医学进步提供了基础.
本综述对于研究循环核酸的研究人员来说是一个有价值的参考资料.

关键词:

申请申请表申请表循环RNA 是一个循环RNA.在CssDNA中使用CssDNA 功能功能功能功能综合合成综合合成

更多相关视频

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

相关概念视频

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

PCR

PCR

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...