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增强修改的聚氨酸电压测量传感器用于定量检测唾液素

Renad S El-Kamel¹, Amany M Fekry¹

¹Cairo University, Faculty of Science, Department of Chemistry, Giza, Egypt.

International journal of biological macromolecules

|July 27, 2024

概括

一种新型的电化学传感器使用碳糊电极 (pTyr/CPE) 上的聚合型氨酸,可以检测唾液氨酸. 这为诊断胃食道逆流性疾病 (GERD) 提供了一种非侵入性方法.

科学领域:

电化学电化学电化学
生物传感器是一种生物传感器.
分析化学分析化学

背景情况:

素是一种关键的消化酶,对于检测胃食道逆流性疾病 (GERD) 至关重要.
当前的诊断方法可能是侵入性的或不舒服的,需要更简单的替代方案.
唾液提供一种非侵入性和可访问的生物流体,用于疾病生物标志物检测.

研究的目的:

开发一个负担得起和敏感的电化学传感器用于唾液素检测.
使用传感器进行GERD的初步诊断.
为了研究聚合型氨酸在碳糊电极 (pTyr/CPE) 上作为氨酸传感器的潜力.

主要方法:

在碳糊电极 (pTyr/CPE) 上通过电聚合合成聚合型氨酸薄膜的合成.
使用循环电压测量 (CV),微分脉冲电压测量 (DPVs),时频测量 (CA) 和电化学阻抗光谱 (EIS) 的电化学表征.
使用扫描电子显微镜 (SEM) 进行表面形态分析.

主要成果:

在pTyr/CPE中,在pH值为2.0的酸盐缓冲器中,表现出对素的敏感电压测量检测.
传感器显示了两个线性范围 (1-20 ng/mL和20-100 ng/mL),检测极限较低 (0.5 ng/mL和0.09 ng/mL).
观察到高选择性,稳定性和1.5秒的快速响应时间.

结论:

pTyr/CPE传感器是通过唾液素检测初步诊断GERD的一个有希望的工具.
该传感器在从喉回流 (LPR) 患者的唾液样本中量化素水平方面表现出可靠的性能.
这种非侵入性的电化学方法为GERD诊断提供了一个可行的替代方案.

关键词:

经过修改的碳糊电极.佩普辛 (Pepsin) 是一种素.聚氨酸氨酸是一种聚氨酸氨酸.这是唾液.传感器传感器传感器

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