血清液体体积对下一代测序的影响:对于优化血清液体样本采集的优化,这是一个重要的进步
Shaham Beg1, Kemin Xu1, James P Solomon1
1Department of Pathology and Laboratory Medicine, New York Presbyterian-Weill Cornell Medicine, New York, New York.
Journal of the American Society of Cytopathology
|July 27, 2024
概括
高达100毫升的血清液体体积 (SFV) 对癌症诊断中下一代测序 (NGS) 的DNA产量产生积极影响. 超过100毫升,SFV不会影响DNA或RNA产量或瘤细胞性.
科学领域:
- 在瘤学瘤学.
- 分子诊断学 分子诊断学
- 基因组学就是基因组学.
背景情况:
- 关于血清液体体积 (SFV) 对恶性病例下一代测序 (NGS) 的影响的数据有限.
- 影响NGS结果的SFV和其他参数需要进一步调查.
研究的目的:
- 调查SFV对恶性溢液样本NGS分析的影响.
- 评估SFV,瘤细胞性和分子数据之间的相关性.
主要方法:
- 评估了来自607名患者的827个血清液样.
- 72个样本接受了NGS分析,评估了输出量,瘤细胞性,DNA/RNA质量和临床病理学数据.
- 胸腔液占样本的56.3%;胃肠/胰腺胆腺癌是最常见的.
主要成果:
- 在SFV和平均瘤细胞性之间没有发现相关性.
- 随着SFV增加至100毫升,DNA产量增加;此外,SFV对DNA或RNA产量没有影响.
- 74.6%的测序样本显示了致癌驱动因素 (KRAS,EGFR,ALK,NTRK1的融合).
- 建议对输液样本进行分子检测,无论瘤细胞性如何.
结论:
- 优化SFV对于改善癌症管理中的诊断和NGS分析至关重要.
- 这项试点研究提供了对用于分子测试的输出样本管理的见解.
关键词:
细胞病理学 细胞病理学它们是DNA DNA DNA DNA.输出 输出 输出 输出国家储备系统 (NGS)这是一个RNARNARNARNARNA.序列化是指测序的使用.血清液体是一种血清液体.时间 量 量 量 量更多相关视频
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