一个用于向致病性细菌物种的细菌素表达平台
Jack W Rutter1, Linda Dekker1, Chania Clare1
1Department of Cell and Developmental Biology, University College London, London, UK.
Nature communications
|July 27, 2024
概括
工程细菌可以分泌细菌,这是抗菌素,以对抗抗生素耐药的病原体. 这项研究开发了一个针对细菌素的向输送平台,显示了对Enterococcus菌株的有效性.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- 细菌素是抗微生物,具有针对抗生素耐药细菌的潜力.
- 工程活生物疗法产品 (eLBPs) 提供有针对性的细菌素输送.
- 由于抗生素耐药性,ESKAPE病原体构成重大威胁.
研究的目的:
- 开发一个模块化平台,从非致病性大肠杆菌中分泌多种细菌素.
- 为了证明工程化细菌素分泌菌株对病原性细菌的疗效.
- 使用Lotka-Volterra方法来建模细菌相互作用.
主要方法:
- 在大肠杆菌中构建了一个模块化的细菌素分泌平台.
- 产生分泌氨酸A (EntA) 和氨酸B (EntB) 的工程菌株.
- 在实验室中使用固体和液体共同培养方法评估抗菌活性.
- 开发了一种Lotka-Volterra模型来模拟细菌种群动态.
主要成果:
- 大肠杆菌菌株成功分泌了功能性氨酸A和氨酸B.
- 在体外表现出针对Enterococcus faecalis和Enterococcus faecium的显著抗菌活性.
- 同时暴露于EntA和EntB延迟了Enterococcus的生长.
- 洛特卡-沃尔特拉模型捕获了竞争对手的菌株相互作用.
结论:
- 开发的平台可以为抗菌剂应用提供有针对性的细菌素生产.
- 工程化细菌素分泌显示出作为对抗抗生素耐药性病原体的战略具有前景.
- 这种方法可以发展为治疗用途的eLBP.
相关概念视频
CRISPR and crRNAs
16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
Antibiotic Selection
52.7K
Overview
52.7K
Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Bacterial Signaling
31.9K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
31.9K
Prokaryotic Transcriptional Activators and Repressors
20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.9K
Lytic Cycle of Bacteriophages
70.6K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.6K


