Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
RNA Structure01:19

RNA Structure

4.7K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.7K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Convergent mechanisms in Wnt and Hedgehog signaling.

Science signaling·2026
Same author

Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement <i>via</i> Single-Cell Genomics.

bioRxiv : the preprint server for biology·2026
Same author

Bioproduction, bioprotection, and biocontainment in multi-kingdom microbial systems with 3D spatial control.

Biofabrication·2026
Same author

Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida.

Nature communications·2025
Same author

Inference of weak-form partial differential equations describing migration and proliferation mechanisms in wound healing experiments on cancer cells.

PLoS computational biology·2025
Same author

Cell-Free-Based Thermophilic Biocatalyst for the Synthesis of Amino Acids from One-Carbon Feedstocks.

ACS synthetic biology·2025

相关实验视频

Updated: Jun 18, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.3K

导向RNA结构设计使组合CRISPRa程序能够用于生物合成分析.

Jason Fontana1,2,3, David Sparkman-Yager1,3, Ian Faulkner1,3

  • 1Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.

Nature communications
|July 27, 2024
PubMed
概括

这项研究引入了一种新的计算方法,用于预测和设计CRISPR激活系统的RNA结构. 这提高了对细菌代谢工程的多基因表达的控制.

更多相关视频

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.5K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.4K

相关实验视频

Last Updated: Jun 18, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.3K
CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.5K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.4K

科学领域:

  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.
  • 分子生物学分子生物学

背景情况:

  • 优化多基因表达对于通过工程代谢途径有效生产化学物质至关重要.
  • CRISPR-Cas转录控制为编程基因表达提供了希望,但受到指导RNA折叠不可预测性的限制.

研究的目的:

  • 为了将指导RNA折叠动力学与E. coli中的CRISPR激活功效相关联.
  • 开发一种用于设计用于CRISPR激活合成RNA组件的预测参数.
  • 为精确的多基因控制设计直角合成CRISPR激活促进器.

主要方法:

  • 与计算动力折叠参数 (rS) 相关的修饰导向RNA (scRNA) 疗效.
  • 利用动力参数进行合成CRISPR激活促进体的前设计.
  • 实施了三种合成促进剂的系统,用于正交基因激活.
  • 采用组合调来对代谢途径表达的3D设计空间进行配置.

主要成果:

  • 在scRNA折叠率和CRISPR激活效率之间发现了强烈的相关性 (rS = 0.8).
  • 成功设计了三种合成的CRISPR激活促进剂,具有正交控制 (>35倍激活).
  • 通过路径优化,证明了里丁和母乳寡糖类产品的可变生产.

结论:

  • 开发的RNA设计方法提高了CRISPR激活系统的可预测性和控制性.
  • 这种方法促进了化学生产代谢途径的组合优化.
  • 在细菌宿主中加速有效的多基因调节程序的常规设计.