使用未经修改的玻璃碳电极对两种可能的抗癌药物候选物的第一次电压分析
Katarzyna Tyszczuk-Rotko1, Katarzyna Staniec1, Krzysztof Sztanke2
1Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031, Lublin, Poland.
Scientific reports
|July 27, 2024
概括
这项研究引入了新的分析方法来量化两种潜在的抗癌药物,即二甲基2 - [2-] 1 - - 4 - 5 - 二 - 1H - 伊米达 - 2 - ) 氨基] 丁二酸盐 (DIHB) 和8 - - 3 - ) - 2 - 6 - 7 - 8四胺基[2,1-c] 1,2,4]三-3,4-二 (HDIT). 使用玻璃碳电极开发的电化学方法在生物样本中提供了敏感和选择性的检测.
科学领域:
- 分析电化学 分析电化学
- 药品分析 药品分析
- 药用化学 医学化学
背景情况:
- 丁甲基2-[2-[]1--4,5-二-1H-伊米达-2-) 化酸 (DIHB) 和8-[]3-化) -2,6,7,8-四化化[2,1-c][1,2,4]三-3,4-二 (HDIT) 正成为有前途的抗癌药物.
- 准确和敏感的分析方法对于这些潜在的治疗化合物的开发和质量控制至关重要.
- 现有的DIHB和HDIT分析程序尚未成熟,需要开发新的量化技术.
研究的目的:
- 开发和验证用于DIHB和HDIT的单独和同时确定的第一个分析程序.
- 使用商用,未经修改的玻璃碳电极 (GCE) 作为差分脉冲电压测量 (DPV) 分析的传感器.
- 建立一种灵敏,选择性和快速量化方法,适合在生物矩阵中分析这些抗癌剂.
主要方法:
- 差分脉冲电压测量 (DPV) 使用未经修改的玻璃碳电极 (GCE) 作为工作电极.
- 研究DIHB和HDIT的电化学行为在乙酸缓冲器 (pH4.5) 中,以确定氧化峰值.
- 优化了实验参数,包括沉积时间,脉冲振幅,扫描速率和修饰器时间,以提高灵敏度.
主要成果:
- DPV分析显示,在优化的缓冲中,DIHB在1.18V和HDIT在0.98V (相对于Ag/AgCl) 的氧化峰值明显.
- 拟议的方法证明了广泛的线性传感范围 (DIHB的1-200 nmol L−1,HDIT的5-200 nmol L−1) 与低的检测极限 (DIHB的0.18 nmol L−1,HDIT的1.1 nmol L−1).
- 该方法具有出色的选择性,可重复性和传感器可重复性,并且在未经先前样本处理的情况下成功应用于尿液参考材料中DIHB和HDIT的分析.
结论:
- 一种使用GCE-DPV的敏感,选择性和快速电化学方法已成功开发用于DIHB和HDIT的量化.
- 这种无修改,环保的方法适合在尿液等复杂矩阵中常规分析这些潜在的抗癌药物.
- 经过验证的方法为进一步研究和开发DIHB和HDIT作为治疗剂提供了宝贵的工具.
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