在Saccharopolyspora erythraea中使用CRISPR/Cas9介导的促进器工程
Yue Zhang1,2, Hui Qi3,4,5, Meng Wang6,7
1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. zhangy@tib.cas.cn.
Methods in molecular biology (Clifton, N.J.)
|July 27, 2024
概括
这项研究介绍了一种CRISPR/Cas9协议,用于Saccharopolyspora erythraea的现场促进器工程. 这种方法可以在不添加额外的DNA序列的情况下在actinomycetes中精确编辑基因.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 在现场促进器工程修改基因表达没有外来DNA.
- 克里斯普尔/Cas9技术促进了有效的,无痕迹的基因组编辑.
研究的目的:
- 描述CRISPR/Cas9介导促进器工程的常规协议.
- 为了在Saccharopolyspora erythraea中实现精确的基因修饰NRRL 23338.8.
主要方法:
- 利用CRISPR/Cas9系统进行针对性基因组编辑.
- 开发了一种S. erythraea.在现场促进器工程中的协议.
主要成果:
- 建立了一种可靠的无痕基因编辑方法.
- 证明了CRISPR/Cas9对促进体修饰的有效性.
结论:
- 该协议提供了一种有效的方法,用于S. erythraea.ea中的促进体工程.
- 这种方法可以适应在其他类型菌体中更广泛的CRISPR/Cas9介导基因编辑.
相关概念视频
01:59CRISPR
50.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.2K
02:53Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K