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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

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相关实验视频

Updated: Jun 18, 2025

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

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一个RNA-Seq数据分析管道

Soham Sengupta1, Rajeev K Azad2

  • 1Center for Pediatric Neurological Disease Research, St. Jude Children's Research Hospital, Memphis, TN, USA.

Methods in molecular biology (Clifton, N.J.)
|July 27, 2024
PubMed
概括
此摘要是机器生成的。

本章详细介绍了可复制RNA-Seq分析管道,从原始数据处理到识别差异表达基因及其功能. 工作流确保可靠的基因表达洞察力,用于生物发现.

关键词:
调整 调整 调整不同基因表达的差异性表达式的量化表达式的量化在RNA-Seqq.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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相关实验视频

Last Updated: Jun 18, 2025

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

7.6K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • RNA测序 (RNA-Seq) 是一种用于测量基因表达的强大技术.
  • 分析RNA-Seq数据需要一个强大的和可重复的计算管道.
  • 了解基因表达模式对于阐明生物过程至关重要.

研究的目的:

  • 为RNA-Seq数据分析提供一个全面的,逐步的管道.
  • 为了使研究人员能够识别差异表达的基因,并执行功能性特征.
  • 为可重复性和定制性提供可访问的脚本和数据.

主要方法:

  • 数据采集和质量控制评估原始测序阅读.
  • 跨样本的基因水平表达量化.
  • 差异基因表达分析用于比较条件并识别重大变化.

主要成果:

  • 建立并描述了一个完整的RNA-Seq分析管道.
  • 该管道有助于识别差异表达的基因.
  • 已识别的基因的功能性特征得到了支持.

结论:

  • 提出的管道为RNA-Seq数据分析提供了一个可靠的框架.
  • 通过在线存储库的可访问性提高了可复制性.
  • 工作流程使研究人员能够深入了解基因表达和生物机制.