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Updated: May 1, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

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Published on: August 18, 2018

使用流细胞计量测试,量化巨细胞吞和酸性化亡细胞的Efferocytosis测试

Xun Wu¹, Ziyi Wang¹, Tyler Shern¹

¹Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.

|July 28, 2024

概括

这项研究提出了一项协议,以测量巨细胞如何吞和酸化亡细胞,这对于解决炎症和保持健康至关重要. 该方法使用光染料和流细胞计来准确量化.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

巨细胞增生对于维持组织平衡和解决炎症至关重要.
巨细胞对亡细胞的有效清除可以防止二次亡,并限制自身免疫反应.

研究的目的:

提供一个详细的方案,以评估巨细胞衰变,特别是吞和随后的亡细胞 (ACs) 的酸化.
为了使研究人员能够使用标准化方法量化细胞化中的这些关键步骤.

主要方法:

制备骨髓衍生的巨细胞 (BMDMs) 和腹巨细胞 (PMs).
使用pH敏感 (pHrodo-Red succinimidyl ester) 和pH不敏感 (Hoechst) 染料对AC进行光标记.
标记的ACs与巨细胞的化,以促进细胞.
通过流细胞计量量化吞和酸化的量化.

主要成果:

该协议允许定量评估食率和巨细胞在细胞分裂期间的酸化能力.
成功的实施使得基于 lysosomal 酸化的早期和晚期的细胞分裂的区别成为可能.

结论:

该协议提供了一种强大且可复制的方法来研究巨细胞衰变.
精确测量细胞,包括酸化,对于理解炎症解消和开发向疗法至关重要.

关键词:

基于细胞的测试流动细胞计量 (Flow Cytometry) 是一种流动细胞计.免疫学免疫学免疫学

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Phagocytosis of Apoptotic Cells

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...