使用滴滴数字PCR测量高阶重复的量化来测量中心数组大小变化的协议

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Updated: Jun 18, 2025

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
09:15

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Published on: July 12, 2022

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Soyeon Showman1, Paul B Talbert2, Yiling Xu2

  • 1Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

STAR protocols
|July 28, 2024
PubMed
概括

这项研究引入了一种新方法,通过量化单细胞中更高阶重复拷贝数来测量心体长度的变化. 这使得研究人员能够研究快速的中间体进化及其分子机制.

关键词:
进化生物学是进化的生物学.基因组学就是基因组学.分子生物学分子生物学

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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