柏柏林通过向IRGM1来抑制炎症,从而缓解性结肠炎
Guibing Meng1, Pengyan Li1, Xuemei Du1
1School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, and State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
柏柏林 (BBR) 向IRGM1,通过抑制PI3K/AKT/mTOR通路来减少性结肠炎 (UC) 中的炎症. 这项研究确定IRGM1是BBR的直接目标,为UC治疗提供了新的治疗见解.
科学领域:
- 药理学 药理学是指药理学的学科.
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 来自Coptis chinensis Franch.的柏柏林 (BBR) 具有与性结肠炎 (UC) 治疗相关的抗炎性质.
- 精确的分子标和BBR在UC中的作用机制在很大程度上仍未确定.
研究的目的:
- 在UC的背景下,确定BBR的特定抗炎点.
- 阐明BBR在UC治疗效果背后的分子机制.
主要方法:
- BBR和Coptis chinensis Franch. 的治疗功效 在UC小鼠模型中评估的提取物.
- 使用氨基酸进行稳定同位素标记,用于基于细胞培养活动的蛋白质分析 (SILAC-ABPP),用于在LPS刺激的RAW264.7细胞中识别BBR标.
- 用于验证BBR-目标相互作用的分子对接,DARTS,分子动力学,CETSA和BLI测试;用于目标敲击的lentiviral转染.
主要成果:
- 在BBR和Coptis chinensis Franch.中 提取物在小鼠中显示出显著的UC缓解.
- SILAC-ABPP将IRGM1确定为直接BBR目标,BBR在体外和体内减少其过度表达.
- BBR抑制PI3K/AKT/mTOR通路,减少LPS诱导细胞中的炎症性细胞因子;IRGM1敲击减轻了这些影响.
结论:
- IRGM1被确定为柏柏林 (BBR) 的直接抗炎标.
- BBR显示出抑制IRGM1表达的能力,无论是体外还是体内.
- 在UC中,BBR通过向IRGM1并随后抑制PI3K/AKT/mTOR通路来发挥其抗炎作用.
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