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JAK和STAT5B调解迁徙对它们自己的挥发性物质的嗅觉反应

Zongyuan Ma¹, Jipeng Liu¹, Lichen Zhang¹

¹College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China; Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.

Insect biochemistry and molecular biology

|July 28, 2024

概括

简氏激酶 (JAK) 和信号转换器和转录激活器 (STAT) 信号通路对于虫的嗅觉偏好至关重要. 这些途径通过影响RNA结合蛋白和多巴胺信号传递来调节对自我气味剂的反应.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
动物行为动物行为

背景情况:

简氏激酶 (JAK) 和信号传感器和转录激活器 (STAT) 信号通路与社会行为和情绪障碍有关.
嗅觉功能障碍是与这些行为相关的症状,但JAK和STAT在嗅觉中的确切作用尚不清楚.
迁徙虫对自己的挥发物有偏好,这使得它们成为研究嗅觉机制的合适模型.

研究的目的:

研究JAK和STAT5B的功能和机制,以调解迁徙虫对自我气味的嗅觉反应.
在嗅觉偏好中阐明连接JAK/STAT5B信号,RNA结合蛋白和多巴胺受体的分子通路.

主要方法:

在虫天线和大脑中使用RNA干扰 (RNAi) 进行基因淘汰.
对天线和大脑中的基因和蛋白质表达的分析.
RNA测序 (RNA-seq) 用于识别下调的转录.
行为测试以评估虫对挥发性物质和特定气味剂 (英多尔,β-离子) 的嗅觉偏好.

主要成果:

JAK和STAT5B在虫的天线和大脑中表达,特别是在天线叶和体中.
在虫中,对JAK和STAT5B的抑制消除了它们对包括英多尔和β-离子子在内的自我气味剂的嗅觉偏好.
JAK/STAT5B信号调节嗅觉,通过调节RNA结合蛋白 hnRNPC 和 SNRPF 的表达,这些蛋白也对嗅觉偏好至关重要.
多巴胺受体1 (DopR1) 信号与JAK/STAT5B通路相互作用,因为DopR1敲击降低了JAK mRNA水平,并且两条通路都需要进行嗅觉偏好.

结论:

在迁徙的虫中,JAK/STAT5B信号在调节对自我气味的嗅觉偏好方面发挥着重要作用.
该JAK/STAT5B通路通过调节RNA结合蛋白 hnRNPC 和 SNRPF 来影响嗅觉.
JAK/STAT5B通路和多巴胺受体1在功能上相互关联,共同需要调节嗅觉偏好自我嗅觉剂.

关键词:

多巴胺是一种多巴胺.杰克·杰克是什么意思迁徙的虫迁徙的虫是一种迁徙的虫.嗅觉行为嗅觉行为在STAT5B中,我们可以看到:

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