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干旱诱导的HpbHLH70增强了干旱耐受性,并可能加速皮塔亚的花诱导

Xiaowei Cai¹, Ling Xiao¹, Aihua Wang²

¹Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Institute of Agro-bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China; Guizhou Key Laboratory of Agro-Bioengineering, Institute of Agro-bioengineering, College of Life Sciences, Guiyang 550025, Guizhou Province, China.

International journal of biological macromolecules

|July 28, 2024

概括

皮塔亚的干旱诱导的HpbHLH70增强了干旱耐受性,并加速了花诱导. 这种转录因子与HpbHLH59相互作用,激活HpSOC1,改善植物的弹性和开花时间.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
农业科学农业科学

背景情况:

花诱导对于水果作物的产量至关重要.
鲜花BHLH (FBH) 转录因子HpbHLH70以前在皮塔亚 (Hylocereus polyrhizus) 中被发现,并与干旱压力有关.
了解HpbHLH70在干旱应对和花发展中的作用,对于提高作物弹性和生产力至关重要.

研究的目的:

调查Pitaya中HpbHLH70的功能,重点关注其在干旱反应和花诱导中的作用.
阐明HpbHLH70影响干旱耐受性和开花时间的分子机制.
探索HpbHLH70作为提高在非生物压力下作物表现的目标的潜力.

主要方法:

GUS记者在转基因Arabidopsis中进行了测试,以确定HpbHLH70表达模式.
在pitaya和转基因Arabidopsis过度表达HpbHLH70.0.的干旱压力治疗.
分析与干旱耐受性相关的生理参数,包括持水能力和ROS清理活动.
酵母二杂交测定验证HpbHLH70与HpbHLH59.5的异构化.
电泳运动转移测定 (EMSA) 和定量实时PCR (qRT-PCR) 来评估HpbHLH70在HpSOC1促进器上的交易活化活动.

主要成果:

HpbHLH70主要在皮塔亚中激活,其表达可通过干旱压力诱导.
过度表达HpbHLH70通过改善水和ROS清理,提高了皮塔亚和阿拉比多普西斯的干旱耐受性.
与野生类型植物相比,过度表达HpbHLH70的转基因阿拉比多普西斯表现出较早的开花.
HpbHLH70与HpbHLH59异构,并直接与花诱导调节器HpSOC1的促进体结合.
过度表达HpbHLH70导致皮塔亚中HpSOC1的表达增加.

结论:

干旱引起的HpbHLH70在提高皮塔亚的干旱耐受性方面发挥着重要作用.
HpbHLH70加速了皮塔亚的花诱导,可能是通过一种涉及与HpbHLH59异体化和HpSOC1.1的交换激活的机制.
研究结果显示,干旱应对和花诱导之间的交叉声由HpbHLH70介导,为改善水果作物的弹性和产量提供了潜在的策略.

关键词:

花蕾诱导诱导花蕾诱导皮塔亚·皮塔亚 (Pitaya Pitaya) 是一个 bHLH转录因子 bHLH转录因子

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