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粘附性侵入性大肠杆菌 (Escherichia coli) LF82破坏了Caco-2单层的紧密连接

Sumei Sha¹, Huijun Gao², Hong Zeng³

¹Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, Key Laboratory of Gastrointestinal Motility Disorders, Clinical Research Center of Gastrointestinal Diseases, Xi'an, Shaanxi Province 710004, PR China.

Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology

|July 28, 2024

概括

粘附性侵入性大肠杆菌 (AIEC) 通过增加透性和损坏紧密结节来破坏肠道上皮屏障. 这一发现突出了AIEC的AIEC.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
免疫学免疫学免疫学

背景情况:

粘附性侵入性大肠杆菌 (AIEC) 经常在患有炎症性肠病 (IBD) 的患者中发现.
通过AIEC影响肠上皮屏障的确切机制尚不清楚.
了解AIEC的作用对于IBD的发病过程至关重要.

研究的目的:

在体外研究AIEC菌株大肠杆菌LF82的作用.
阐明AIEC对肠上皮质屏障完整性的影响.
探索Th17在AIEC调解过程中的参与.

主要方法:

通过表皮电阻和跨表皮电阻 (TEER) 测量来评估表皮屏障完整性.
透度使用膜-血清流量率评估的路西弗黄色.
通过免疫光和西部斑点分析紧结蛋白ZO-1和Claudin-1的表达和分布;用ELISA测量细胞因子分泌.

主要成果:

AIEC感染显著降低了TEER,并以时间和剂量依赖的方式增加了屏障透性.
AIEC降低了ZO-1和Claudin-1紧结蛋白的表达并改变了它们的分布.
感染诱导了促炎性细胞因子的分泌,包括TNF-α和IL-17.

结论:

AIEC菌株大肠杆菌LF82明显增加了肠道上皮质屏障的透性.
AIEC 破坏了关键紧密的连接点,损害了屏障功能.
这些发现表明,AIEC会加剧肠道的炎症反应.

关键词:

粘附性侵袭性大肠杆菌 (Escherichia coli) 是一种具有粘附性侵袭性的疾病.炎症性肠病是一种炎症性肠病.介质蛋白-17 介质蛋白-17 介质蛋白肠道的微生物群.

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