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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Jun 18, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

从分子到教室:单分子定位显微镜的全面指南

Guangjie Yan¹, Yuteng Zhang¹, Alexis Allamprese¹

¹Department of Chemistry, University of Houston, Houston, Texas 77204.

Journal of chemical education

|July 29, 2024

概括

本研究介绍了单分子局部化显微镜 (SMLM) 概念和一个用户友好的应用程序,以简化学生的超高分辨率成像. 该工具有助于理解高级显微镜技术的图像拟合和质量因素.

科学领域:

生物物理学的生物物理.
显微镜的使用方法
细胞生物学细胞生物学

背景情况:

单分子局部化显微镜 (SMLM) 提供高分辨率的细胞成像.
对于学生来说,SMLM复杂的生物物理原理构成了学习挑战.

研究的目的:

为学生阐明SMLM的基本概念.
开发一个可访问的应用程序,简化SMLM原则和图像分析.

主要方法:

关于SMLM生物物理学的理论解释.
开发一个直观的图形用户界面 (GUI) 应用程序.
专注于SMLM中的图像匹配和质量确定.

主要成果:

一个简化的SMLM理论框架.
一个交互式应用程序,展示超高分辨率图像的合适性.
确定影响SMLM图像质量的关键因素.

结论:

结合理论和实践的方法提高了学生对SMLM的理解.
开发的应用程序使学生能够执行和理解超分辨率显微镜实验.

关键词:

图像定量化图像定量化图像形成图像形成点差函数的点差函数空间分辨率空间分辨率波浪光学是一种波浪光学.

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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

相关实验视频

Last Updated: Jun 18, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

有助于探索超出衍射极限的细胞结构.