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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
585
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: Jun 18, 2025

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

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开发用于低输入向蛋白质组的快速测试,使用多功能线性离子陷.

Brian Searle1, Ariana Shannon1, Rachael Teodorescu1

  • 1Ohio State University.

Research square
|July 29, 2024
PubMed
概括
此摘要是机器生成的。

本研究介绍了一种具有成本效益的混合四极-线性离子陷 (LIT) 质谱工作流程,用于向蛋白质组学. 它可以从全球数据独立获取中快速开发测试,为有限的细胞群体民主化先进的蛋白质组分析.

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相关实验视频

Last Updated: Jun 18, 2025

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 质谱测量质量谱测量
  • 细胞生物学 细胞生物学

背景情况:

  • 在蛋白质组学中,通常需要高质量精度仪器来研究有限的细胞群.
  • 由于低质量精度测量,三重四极仅限于向蛋白质组学.
  • 线性离子陷 (LIT) 为目标和全球蛋白质组学提供了一种多功能,具有成本效益的选择.

研究的目的:

  • 描述一种工作流程,用于开发针对性蛋白质组学测试,从使用混合四极-LIT仪器的全球数据独立采集 (DIA) 测量.
  • 为了证明这个工作流程的能力,而不需要高质量准确度.
  • 展示该仪器用于分析有限的细胞群和低水平蛋白质的实用性.

主要方法:

  • 使用了一种新的混合四极-线性离子陷 (LIT) 仪器.
  • 开发了一种自动化的软件方法来安排并行反应监测 (PRM) 试验.
  • 执行全球数据独立获取 (DIA) 进行测试开发.
  • 在1 ng背景蛋白质组中的量化低水平蛋白质.

主要成果:

  • 在一个匹配的矩阵背景中,在三个数量级实现了一致的量化.
  • 对于像转录因子和细胞因子这样的低水平蛋白质,在没有稳定的同位素标记标准的情况下,证明了低于两次数的定量线性.
  • 从1ng样本中使用基于LIT的蛋白质组学和高维流细胞计,显示了CD4+和CD8+T细胞子集中的蛋白质之间的一致性.

结论:

  • 混合四极LIT仪器为目标蛋白质组学提供了一个经济的解决方案.
  • 这种工作流使质谱学在各种实验室环境中实现民主化,特别是对于有限的样本大小.
  • 该方法可以对细胞研究中至关重要的低丰度蛋白质进行敏感的量化.