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Updated: Jun 18, 2025

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切除内皮葡萄糖的成分内素会导致淋巴细胞结构和功能受损

Zhengping Hu, Issahy Cano, Fengyang Lei

bioRxiv : the preprint server for biology

|July 29, 2024

概括

在小鼠中,内素 (EMCN) 缺乏会损害淋巴膜过屏障的功能,导致白蛋白尿和细胞损伤. 这凸显了EMCN在维护脏健康和内皮细胞-细胞通信方面的关键作用.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
血管生物学血管生物学
分子医学是分子医学.

背景情况:

内素 (EMCN) 是内皮糖的成分,在球体中高度表达.
EMCN在调节血管内皮生长因子 (VEGF) 活性方面发挥作用,具有抗炎性质.

研究的目的:

使用淘汰赛小鼠模型研究全球EMCN缺乏的表型和功能后果.

主要方法:

生成的全球EMCN淘汰赛小鼠 (EMCN-/-).
分析了脏骨髓细胞透,膜过屏障超结构和尿蛋白水平.
在质内皮细胞和细胞上进行RNA-seq,以评估转录变化.
检查了关键脏和内皮标记物的蛋白质表达.

主要成果:

在EMCN-/-小鼠中,骨髓细胞透和VCAM-1表达的增加.
在EMCN-/-小鼠中观察到白蛋白尿和 podocyte 脚过程消失.
RNA-seq揭示了细胞-细胞粘附,MAPK/ERK和VEGF信号通路的改变,并减少了多多的表达.

结论:

在保持淋巴膜过屏障完整性和功能方面,EMCN至关重要.
通过内皮细胞-内皮细胞交叉连接,EMCN支持质内皮细胞平衡和 podocyte 功能.

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