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使用高度和的转子子子库,识别了Schaalia odontolytica中必不可少的基因

Joseph K Bedree^1,2, Jacob Bourgeois³, Pooja Balani^2,4

¹Section of Oral Biology, Division of Oral Biology and Medicine, School of Dentistry, University of California-Los Angeles, Los Angeles, CA, 90095.

bioRxiv : the preprint server for biology

|July 29, 2024

概括

这项研究开发了高通量转位子测序 (Tn-seq) 来识别Schaalia odontolytica中必不可少的基因,这是虫-寄生虫关系中的关键合作伙伴. 研究人员确定了203个基本基因,为研究这种独特的细菌相互作用提供了先进的遗传工具.

科学领域:

微生物学微生物学
基因组学就是基因组学.
细菌遗传学细菌遗传学

背景情况:

在Nanosynbacter lyticus (Patescibacteria) 和Schaalia odontolytica之间的病-寄生关系需要先进的遗传工具进行全面的研究.
目前对Nanosynbacter lyticus的遗传工具有限,这阻碍了对其与S. odontolytica的强制性相互作用的深入分析.

研究的目的:

通过开发和应用高通量转位子插入测序 (Tn-seq) 来扩大Schaalia odontolytica的遗传工具集.
在实验室条件下使用Tn-seq.识别S. odontolytica中必不可少的基因.

主要方法:

为S. odontolytica生成一个高度和的Tn-seq库,产生大约66万个独特的插入突变.
在体外,Tn-seq的应用用于选S. odontolytica生存和生长所必需的基因.

主要成果:

在S. odontolytica基因组中识别了203个假定必不可少的基因,占其总基因的10.5%.
该研究平均每2-3个核酸进行一次插入,这表明图书馆和度很高.

结论:

开发的Tn-seq方法为S. odontolytica的遗传剖析提供了一个强大的新工具.
这项研究显著提高了对Patescibacteria和S. odontolytica之间强制性关系的遗传基础的理解.

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