相关实验视频

Updated: Jun 18, 2025

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

通过流动细胞计量对牙买加果蝙蝠淋巴细胞的歧视

Bradly E Burke, Savannah M Rocha, Corey Campbell

bioRxiv : the preprint server for biology

|July 29, 2024

概括

研究人员开发了新的工具来研究蝙蝠免疫力. 这些单克隆抗体有助于在牙买加果蝙蝠中识别T细胞和B细胞,促进传染病研究,并了解蝙蝠如何在没有疾病的情况下控制病毒.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
比较生物学的比较生物学

背景情况:

蝙蝠是动物传播病毒的天然储存库,但由于缺乏研究工具,它们的免疫反应仍然不明.
牙买加果蝙蝠 (Artibeus jamaicensis) 作为研究流感和冠状病毒的模型.

研究的目的:

为研究牙买加果蝙蝠的适应性免疫反应开发免疫试剂.
在这种蝙蝠物种中识别和产生针对关键淋巴细胞标记物的单克隆抗体.

主要方法:

选商用单克隆抗体,以检测与蝙蝠淋巴细胞的交叉反应.
产生混合体,产生针对蝙蝠CD3γ,CD4和CD8α的特定单克隆抗体.
使用流细胞计,免疫光显微镜和PCR验证的抗体特异性.

主要成果:

识别了用于T和B细胞识别的交叉反应单克隆抗体.
成功生成了针对蝙蝠CD3γ,CD4和CD8α的新型单克隆抗体.
发现了一种不寻常的淋巴细胞群体,共同表达B细胞 (CD19) 和T细胞 (CD3) 标记物.

结论:

开发的单克隆抗体是蝙蝠免疫系统详细分析的关键试剂.
这些工具将有助于更深入地了解蝙蝠如何在不发展疾病的情况下管理病毒感染.

更多相关视频

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...