Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Gene syntax defines supercoiling-mediated transcriptional feedback.

Science (New York, N.Y.)·2026
Same author

The Rayleigh Quotient and Contrastive Principal Component Analysis II.

bioRxiv : the preprint server for biology·2026
Same author

Hybrid crosses reveal a cell-type-specific landscape of mouse regulatory variation.

bioRxiv : the preprint server for biology·2026
Same author

Peptide signaling in the paraventricular thalamus contributes to disrupted adult reward behaviors after early-life adversity.

bioRxiv : the preprint server for biology·2026
Same author

T cell development from expanded hematopoietic progenitors reveals initiation control by <i>Lmo2</i> and Flt3L priming.

Science immunology·2026
Same author

Impact of peripheral nerve blocks on chronic opioid use after elective total knee arthroplasty from a large US claims database: impact of intraoperative opioids. Comment on Br J Anaesth 2025; 134: 1779-83.

British journal of anaesthesia·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 18, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K

使用lr-kallisto进行长读测序转录组量化.

Rebekah K Loving1, Delaney K Sullivan1,2, A Sina Booeshagi3

  • 1Division of Biology and Biological Engineering, California Institute of Technology, USA.

bioRxiv : the preprint server for biology
|July 29, 2024
PubMed
概括
此摘要是机器生成的。

准确的全长转录异形量化现在可以通过长读序列数据实现. 新的lr-kallisto工具,通过外体捕获进行了改进,可以快速准确地定量RNA丰度.

更多相关视频

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.2K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

719

相关实验视频

Last Updated: Jun 18, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.2K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

719

科学领域:

  • 基因组学和生物信息学
  • 分子生物学分子生物学
  • 文字转录学 (Transcriptomics) 是一个学科.

背景情况:

  • 高通量短读测序使得常规基因水平的RNA丰度定量成为可能.
  • 准确和负担得起的全长转录异型的量化仍然是一个重大挑战.
  • 转录单体的复杂性和遗传变异使量化工作复杂化.

研究的目的:

  • 为了应对使用长读序列数据的最终全长转录异形量化挑战.
  • 开发和验证一个计算工具,用于准确的长读RNA量化.
  • 评估外体捕获对长读量化准确性的影响.

主要方法:

  • lr-kallisto的开发, kallisto的适应用于长时间读取的测序数据.
  • 利用牛津纳米孔 (ONT) 测序数据用于方法开发和测试.
  • 整合了外汇捕获技术,以评估量化准确度的改进.

主要成果:

  • 证明了长时间读取的RNA测序数据的快速和准确量化是可行的.
  • 表明外体捕获显著提高了长读数据量化的准确性.
  • lr-kallisto成功地将现有的量化方法适用于长期阅读的技术.

结论:

  • 长读测序技术与lr-kallisto相结合,可以实现常规的最终同型量化.
  • 外体捕获是一个有价值的策略,可以提高长时间读取的转录量化的精度.
  • 这项工作克服了长时间读取数据分析的关键生物信息学挑战,以解决转录异形.