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Updated: May 12, 2026

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开发逆转录酶-重组酶聚合酶放大 (RT-RPA) 试验,以快速检测大红奇尔克病毒

P Malavika1, A I Bhat1, M Greeshma1

  • 1ICAR-Indian Institute of Spices Research, Kozhikode, Kerala 673012 India.

Virusdisease
|July 29, 2024
PubMed
概括

开发了一种新的快速检测方法,用于大红奇尔克病毒 (LCCV). 这种逆转录酶-重组酶聚合酶放大 (RT-RPA) 试验对于在植物中识别LCCV具有高度敏感性和准确性.

关键词:
诊断 诊断 诊断 诊断 诊断这是RT-PCR.RT-RPA 其他类型敏感度 敏感度 敏感度

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...