SLC38A9调节了SARS-CoV-2病毒进入的过程.
Gaurav Datta1, Neda Rezagholizadeh1, Wendie A Hasler1
1Department of Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58203, USA.
iScience
|July 29, 2024
概括
这是一种尖蛋白质.
科学领域:
- 病毒学和细胞生物学
- 病毒进入的分子机制
背景情况:
- SARS-CoV-2 病毒进入依赖于尖端 (S) 蛋白分裂成 S1 和 S2 子单元.
- 素分裂暴露了S1上的多基因图案,对感染和传播至关重要,但其确切作用尚不清楚.
研究的目的:
- 阐明S1多基因图案对SARS-CoV-2感染有所贡献的机制.
- 为了确定与S1动机相互作用并调解病毒进入的宿主因素.
主要方法:
- 研究了S1多基因和宿主细胞蛋白之间的相互作用.
- 使用基于细胞的测定 (Calu-3,U87MG,Caco-2,A549) 与伪SARS-CoV-2.
- 进行了SLC38A9的淘汰实验,以评估其在病毒入口和内分泌体功能中的作用.
主要成果:
- 这种S1多基因图案直接与SLC38A9相互作用,SLC38A9是内解体体中的氨酸传感器.
- SLC38A9敲击抑制了S1诱导的内酶体脱酸.
- 阻止S1-SLC38A9相互作用可以防止伪SARS-CoV-2进入多种细胞类型.
结论:
- 发现了一种新的机制,S1与内酶体SLC38A9相互作用,以破坏内酶体酸化.
- 这种S1-SLC38A9的相互作用促进了SARS-CoV-2从内分泌体中逃脱,增强了病毒的进入和感染.
- 针对S1-SLC38A9相互作用可能提供一种抑制SARS-CoV-2的策略.
相关概念视频
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Single Nucleotide Polymorphisms-SNPs
14.9K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
14.9K
Receptor-mediated Endocytosis
6.0K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.0K
Rous Sarcoma Virus (RSV) and Cancer
5.0K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.0K


