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分析Colo320细胞中纳普洛克森激活细胞死亡途径的分析

Andrew Chen^1,2, Wei Zhu^2,3, Rian Goding^2,4

¹College of Arts and Sciences at The State University of New York at Stony Brook, Stony Brook, NY 11794, USA.

Molecular and clinical oncology

|July 29, 2024

概括

纳普洛克森 (NS) 通过显著降低癌细胞存活率和迁移,显示出对抗结直肠癌的前景. 这种非类固醇抗炎药物还降低了参与癌症扩散和抗药性关键基因的下调.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

大肠直肠癌 (CRC) 是癌症相关死亡的主要原因.
目前的CRC治疗通常涉及严重的副作用,需要探索替代疗法.
非类固醇抗炎药物 (NSAIDs) 具有潜在的化学预防性.

研究的目的:

为了研究纳普罗森 (NS) 对结直肠癌的化学预防潜力.
阐明NS的抗癌作用背后的分子途径和机制.
评估NS对结直肠癌细胞活力,迁移和特定基因表达的影响.

主要方法:

治疗癌症Colo320和健康的CCD-18细胞,使用不同度的纳素 (NS).
测量卡斯帕-3活性测试以测量亡.
细胞生存和迁移测定.
RNA测序以识别基因表达变化.
与酶相关的免疫吸收试验 (ELISA) 用于验证.

主要成果:

在Colo320细胞中,NS显著增加了caspase-3活性 (300%) 并降低了细胞存活率 (-72.888%),但对CCD-18细胞没有显著影响.
NS显著降低了Colo320细胞迁移 (86.58%).
RNA测序揭示了NS处理的Colo320细胞中MUC5B,S100A9和MUC5AC基因的显著下调,这些基因与CRC增殖,干性和耐药性有关.

结论:

纳普罗森 (NS) 显示出显著的抗结肠直肠癌活性.
通过诱导亡,抑制细胞迁移和降低关键癌症相关基因的调节,NS发挥其作用.
这项研究确定了NS抗结直肠癌疗效的新型分子机制,突出了其治疗潜力.

关键词:

灭症 (apoptosis) 是一种死亡的过程.在CCD-18中.颜色32020 颜色320 颜色320 结肠直肠癌是什么意思纳普罗素 (naproxen) 是一种药物.非类固醇抗炎药物是指非类固醇抗炎药物.

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