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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique
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功能性光成像探针在大量实践中的使用:实际和技术含义.

Yan Jiang1, Raul Vazquez-Reyes2, Afrin Kamal2

  • 1Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Redwood, CA 94063, United States. yjiang24@stanford.edu.

World journal of gastrointestinal endoscopy
|July 29, 2024
PubMed
概括

功能性光膜成像探头 (FLIP) 的使用正在增加,用于食道疾病,如食. 麻醉选择对运动性评估产生影响,突出显示在临床实践中需要对技术方面的认识.

关键词:
内镜检查是指内镜检查.消化道中的食道功能性光成像探针探测器胃肠病学 胃肠病学移动性 移动性 移动性

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科学领域:

  • 胃肠病学 胃肠病学
  • 食道生理学 食道生理学
  • 医疗器械技术 医疗器械技术

背景情况:

  • 功能性光膜成像探针 (FLIP) 是FDA批准的食道疾病.
  • 大规模采用和在大量实践中的实用性仍然不清楚.

研究的目的:

  • 分析FLIP使用的大规模临床数据.
  • 提供FLIP程序的技术方面的见解.

主要方法:

  • 在一个学术医疗中心对398个FLIP手术进行了回顾性分析.
  • 收集关于人口统计和程序细节的数据.
  • 统计分析包括千平方,t测试和回归模型.

主要成果:

  • 随着时间的推移,FLIP病例有所增加,特别是在食障碍和GERD方面.
  • 转向更长的FLIP气球导管用于诊断 (70.4%).
  • 麻醉使用与改变的食道运动模式相关.

结论:

  • 随着指标的扩大,FLIP的使用量正在增长.
  • 供应商必须考虑技术因素,包括镇静,以准确评估运动性.
  • 有限的规范性数据需要仔细解释FLIP结果.