相关实验视频

Updated: Jun 18, 2025

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

肠道细胞系HT29与Lacticaseibacillus共同培养的表观遗传景观

Anunay Sinha^1,2,3, Sanjeev Khosla^1,3

¹Centre for DNA Fingerprinting & Diagnostics, Hyderabad, 500039, India.

|July 29, 2024

概括

乳酸菌改变了人类肠道HT29细胞的表观遗传特征. 这种益生菌细菌修改了组素和m6AmRNA标记,影响了特定的遗传通路和基因表达.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
微生物学微生物学
细胞生物学细胞生物学

背景情况:

人的肠道微生物群在健康和疾病中起着至关重要的作用.
在表观遗传层面上了解宿主-微生物相互作用对于开发新型治疗策略至关重要.

研究的目的:

研究Lacticaseibacillus共同培养对HT29人类肠道细胞表观遗传修饰的影响.
确定特定的表观遗传变化及其与基因表达变化的相关性.

主要方法:

在HT29细胞与Lacticaseibacillus的共同培养中.
通过使用ChIP-Seq和西部涂抹,分析基因组修饰 (H3K9ac,H3K4me3,H3K9me3).
通过直接mRNA测序评估m6AmRNA甲基化.
使用LC-MS识别Lacticaseibacillus的分泌体.

主要成果:

同培养导致H3K9ac和H3K4me3的全球丰富,以及HT29细胞中H3K9me3的枯竭.
表观遗传标记显示,基因转录起点 (TSS) 和上游区域的分布发生了变化.
在与特定基因通路相关的mRNA中,m6A甲基化模式被修改.
发现Lacticaseibacillus HU蛋白与海斯H3相互作用.

结论:

乳酸菌可以诱导人类肠道细胞的显著表观遗传变化.
这些修改影响了特定的遗传通路,这表明了益生菌介导的基因调节机制.
这些发现突显了Lacticaseibacillus在调节宿主细胞表观遗传学方面的潜力.

关键词:

表观遗传学是指表观遗传学.基质子的修改基质子的修改宿主-微生物相互作用相互作用乳酸菌种 (lactobacillus) 是一种乳酸菌.m6A甲基化的情况.

更多相关视频

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Published on: July 4, 2018

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...