相关实验视频

Updated: Jun 18, 2025

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

脂质调节元件与CD44 相互作用在牛脂肪细胞分化和脂质新陈代谢中的线粒体生物能量学上

Guanghui Li¹, Xibi Fang¹, Yinuo Liu^1,2

¹College of Animal Science, Jilin University, 5333 Xi An Road, Changchun, Jilin 130062, People's Republic of China.

Journal of agricultural and food chemistry

|July 29, 2024

概括

微RNA-199a-3p通过向CD44基因来调节牛体脂肪细胞的分化. 这影响脂质新陈代谢,脂肪沉积和能量通路,为牛肉肥胖提供了洞察力.

科学领域:

动物科学动物科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

CD44基因影响哺乳动物的胰岛素抵抗和脂肪积累.
关于CD44在牛脂代谢和脂肪生成中的作用的研究有限.

研究的目的:

在牛前脂肪细胞分化过程中研究CD44和miR-199a-3p表达.
阐明 miR-199a-3p 和 CD44.4 之间的调节关系.
确定CD44在牛脂肪细胞脂质代谢中的功能.

主要方法:

对CD44和miR-199a-3p表达在分化牛前脂细胞中的分析.
路西法雷斯记者分析证实了miR-199a-3p对CD44的直接向.
基因敲除和多组学分析 (包括脂组学和转录组学).

主要成果:

CD44是miR-199a-3p的一个直接目标.
调节miR-199a-3p或CD44的减少加速了分化,增加了脂质滴和甘油三,改变了脂肪酸代谢.
CD44的淘汰影响了脂肪细胞特异性基因 (LPL,FABP4) 和脂质代谢物.
多组学揭示了丰富的能量代谢途径 (MAPK,cAMP,信号) 和改变的线粒体呼吸和糖解.

结论:

miR-199a-3p调节牛脂肪细胞中的CD44,影响脂解,糖解,线粒体呼吸,脂肪沉积和脂滴组成.
CD44在牛脂代谢中起着重要的调节作用.
这些发现为肉牛的脂肪生成和脂质代谢提供了分子洞察力.

关键词:

在CD4444中,我们可以看到CD44.牛肉脂肪细胞的使用.脂质新陈代谢脂质新陈代谢线粒体能量代谢的能量代谢

更多相关视频

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

相关概念视频

Overview of Lipid Metabolism

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...

Cholesterol: Significance and Regulation

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...