在精子中的聚类蛋白表达和分布作为男性生育能力的预测因素
María Hernández-Herrador1, García-Aranda Marilina2,3,4,5, María Luisa Hortas6
1Reproduction Unit, Hospital Universitario Costa del Sol, Marbella, Spain.
Molecular reproduction and development
|July 29, 2024
概括
集群蛋白 (CLU) 水平和精子中的体染色是男性生育能力的新预测因素. 这些通过mRNA分析和免疫染色检测识别的标志物,独立地表明怀孕失败风险,超过了传统的精液分析.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 安德罗学与人类学
背景情况:
- 集群蛋白 (CLU) 是男性生殖液中的关键糖蛋白,对精子生成和精子成熟至关重要.
- 传统的精液分析在准确评估男性受精能力方面存在局限性.
- 在正常和异常的精子之间存在CLU丰度的变化.
研究的目的:
- 评估精子中的mRNA-CLU水平和蛋白质分布,作为精子质量的潜在标记.
- 评估CLU标志物对男性生育能力和体外受精 (IVF) 结果的预测价值.
- 为了研究CLU表达,精子成熟度和传统的精子图参数之间的关系.
主要方法:
- 一项多中心研究涉及90名接受试管婴儿治疗的患者和36名肥沃的男性.
- 使用定量实时PCR (qPCR) 来测量mRNA-CLU水平.
- 免疫抑制被用来分析精子中的CLU蛋白分布.
- 关联分析CLU标记物,试管婴儿结果和精子图变量之间的相关性分析.
主要成果:
- 精子色体中的CLU染色和升高的mRNA-CLU水平被确定为妊娠失败的独立风险因素 (p<0.01).
- 这些CLU标志物预测了怀孕失败,而不依赖于传统的分红图变量.
- 在生育能力强的男性和患有变异精子图或不孕症的患者之间,CLU的丰度和分布有所不同.
结论:
- 在男性生育能力评估中,mRNA-CLU水平和体CLU免疫染色是怀孕失败的新型独立预测因素.
- 结核细胞分布模式与精子成熟度以及在异常精液分析中观察到的变化相关.
- 精子细胞瘤,特别是它的分泌异型,是成熟过程中形成的精子蛋白池的重要组成部分.
相关概念视频
Infertility in Males
256
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
256
Spermatogenesis
102.3K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.3K
Sperm Structure and Semen Composition
1.7K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
1.7K
Sperm Transport
731
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
731
Fertilization
70.9K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
70.9K


