基于UPLC-MS/MS与多模电离的多模电离的基底下丘脑下垂体淋巴轴18类固醇的同时测定
Yinan Zhang1, Guanghui Wang1, Yongjian Shao1
1Hangzhou Normal University, 311121, Hangzhou, Zhejiang, China. 20080099@hznu.edu.cn.
The Analyst
|July 29, 2024
概括
一种新的超高性能液态色谱法准确地测量了血清中的18种类固醇激素. 这种敏感的技术有助于了解各种生物体中对下丘脑-垂体-腺轴的环境影响.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 内分泌学 在内分泌学.
背景情况:
- 环境污染物可以通过改变类固醇激素水平来破坏内分泌系统.
- 精确量化多个类固醇激素对于评估这些干扰至关重要.
- 现有的方法可能需要大量的样本或与特定的分析物 (如胆固醇) 进行斗争.
研究的目的:
- 开发一种简单,灵敏,同时超强的液体染色学-并联质谱法 (UPLC-MS/MS) 方法.
- 量化18个关键的类固醇激素参与下丘脑-垂体-淋巴结核 (HPG) 轴.
- 为了能够客观地评估环境负荷对类固醇激素形状的影响.
主要方法:
- 开发一种使用液体提取和多模电喷/大气压化学电离 (ESCi) 源的UPLC-MS/MS方法.
- 对包括胆固醇在内的18种类固醇激素的质谱,液相和预处理步骤的优化.
- 使用最小血清体积 (100μL) 验证该方法的线性 (r2 > 0.99),回收 (76.22-113.66%) 和精度 (7.52-1.14%).
主要成果:
- 18种类固醇激素的同时成功测定:胆固醇,阿尔多,皮质素,水皮质素,21-脱氧皮质醇,皮质,11-脱氧皮质醇,安德罗二,雌激醇,雌激素,2-甲基二,21-基,17-α基,,脱二,孕激素,二二和孕激素.
- 该方法表现出高灵敏度,线性,回收和精度.
- 该方法成功地应用于来自 *Mus musculus*, *Carassius auratus*, *Rana catesbeiana Shaw* 和 *Rana nigromaculata* 的血清样本.
结论:
- 开发的UPLC-MS/MS方法为同时进行类固醇激素分析提供了高效和敏感的工具.
- 这种方法需要很小的血清量,可以量化像胆固醇这样具有挑战性的类固醇.
- 它对于评估各种物种对HPG轴的环境影响非常有价值.
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