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一个AP2/ERF成员LlERF012通过激活中的HSF通路来赋予热耐受性

Ting Li^1,2, Ze Wu^1,2, Yinyi Zhang^1,2

¹Key Laboratory of Landscaping Agriculture, Ministry of Agriculture and Rural Affairs/Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing, China.

Plant, cell & environment

|July 29, 2024

概括

莉莉莉莉莉莉莉莉莉莉莉莉莉莉莉

科学领域:

植物分子生物学植物分子生物学
植物应激生理学植物应激生理学
转录因子调节转录因子调节

背景情况:

热应激转录因子 (HSF) 对于植物热应激反应 (HSR) 是至关重要的.
高温压力转录因子 (HSF) 的监管网络,特别是热应力转录因子B (HSFB) 成员,是复杂的,并未完全理解.
了解这些网络对于改善植物耐热性至关重要.

研究的目的:

阐明LlERF012-LlHSFA1模块在百合花 (Lilium longiflorum) 热应激反应中的作用.
确定LllERF012作为LllHSFB1的直接转激活剂,并研究其与LllHSFA1.1的相互作用.
探索LlERF012-LlHSFA1模块如何调节中的其他HSF成员 (HSFA2,HSFA3A,HSFA3B).

主要方法:

基因表达分析 (过度表达和沉默) 在转基因阿拉比多普西斯和百合.
蛋白质与蛋白质相互作用测试 (例如,酵母双混合,共免疫沉).
促进体结合试验 (例如,电泳运动转移试验,双露西法酶记者试验).

主要成果:

LlERF012 作为百合花和阿拉比多的热耐受性的积极调节者.
LlERF012 直接激活了 LlHSFB1 和 LlHSFA1.1 的转录.
LlERF012和LllHSFA1之间的相互作用增强了LllERF012的交易活化和DNA结合活动,促进了HSFA2,HSFA3A和HSFA3B的表达.

结论:

LlERF012是百合花热耐受性的关键调节剂,通过 LlERF012-LlHSFA1 模块发挥作用.
该模块协同调节HSF通路,包括A类和B类成员,以协调工厂的热应激反应.
这些发现为复杂的HSF监管网络提供了重要的洞察力,以增强植物耐热性.

关键词:

百合花长 (Lilium longiflorum) 是一种长的植物.在 LlERF012-LlHSFA1 相互作用中.一个LlHSFB1一个.热应激是一种热应激.

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