Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Histone Modification02:32

Histone Modification

13.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.2K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Case report of a rare TCF3::BEND2-fused primary intracranial neuroepithelial neoplasm in a female child.

Brain tumor pathology·2026
Same author

Cyclosporine A promotes renal fibrosis through activating endoplasmic reticulum stress by targeting miR-212-5p/ATF6 axis.

Journal of translational medicine·2026
Same author

ZDHHC9-Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus-Induced Erectile Dysfunction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Long-term survival in a patient with non-small cell lung cancer harboring <i>KRAS</i> G13C and <i>TP53</i> co-mutations: case report and literature review.

Frontiers in medicine·2026
Same author

Comparative machine learning to predict acute kidney injury in traumatic brain injury: a MIMIC-IV cohort with SHAP interpretation.

Frontiers in medicine·2026
Same author

Astrocyte-Targeted Nanotherapeutics Modulate Iron Homeostasis in Cerebral Amyloid Angiopathy by Restoring the Astrocytic Trafficking Hub Function.

ACS nano·2026

相关实验视频

Updated: Jun 18, 2025

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K

HDAC8通过去乙化ETS1来增强HIF-2α的功能,以降低ccRCC中TKI的敏感性.

Kang Qian1,2,3, Wei Li1,2, Shangqing Ren4

  • 1Department of Urology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|July 29, 2024
PubMed
概括

清细胞细胞癌 (ccRCC) 对氨酸激酶抑制剂 (TKI) 的耐药性是一个挑战. 这项研究确定HDAC8是TKI耐药性的关键参与者,为ccRCC治疗提供了新的治疗点.

关键词:
在ETS1中,ETS1是ETS1.在HDAC8中,它是HDAC8.这就是TKI.ccRCCCC 在线观看

更多相关视频

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

818
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K

相关实验视频

Last Updated: Jun 18, 2025

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

818
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K

科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 对氨酸激酶抑制剂 (TKIs) 的耐药性限制了清细胞细胞癌 (ccRCC) 的存活率.
  • 了解获得耐药性的机制对于开发有效的ccRCC疗法至关重要.

研究的目的:

  • 为了确定参与ccRCC对TKIs耐药性的新目标.
  • 阐明HDAC8在TKI耐药性中的作用背后的分子机制.
  • 开发策略来克服ccRCC中的TKI阻力.

主要方法:

  • 基于CRISPR的全基因组查,以确定与耐药性相关的基因.
  • 分子测试用于研究蛋白质相互作用和翻译后修饰 (乙化,酸化).
  • 基于HDAC8的蛋白质溶解向奇默体 (PROTACs) 的合成和评估.

主要成果:

  • 鉴定出HDAC8是降低ccRCC对苏尼蒂尼布敏感性的关键调解剂.
  • HDAC8通过去乙ETS1,增强ETS1/HIF-2α复合物的活性来促进TKI耐药性.
  • 抑制HDAC8上调调节NEK1,从而进一步促进ETS1/HIF-2α相互作用.
  • 通过STAT3抑制,TKI治疗增加了HDAC8的表达,从而导致获得的耐药性.
  • 合成了HDAC8-in-PROTACs以降解HDAC8并克服TKI抵抗.

结论:

  • 在ccRCC中,HDAC8在获得对TKI的耐药性方面发挥着至关重要的作用.
  • 针对HDAC8,特别是通过PROTACs,为克服ccRCC中的TKI耐药性提供了一个有希望的治疗策略.
  • HDAC8是针对ccRCC向治疗的潜在治疗候选者.