逆氧化TCA (roTCA) 用于通过来自盐湖的Acidimicrobiia菌株进行碳固定
Lei Gao1,2, Lan Liu3, Ai-Ping Lv3
1Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, PR China.
The ISME journal
|July 29, 2024
概括
这项研究揭示了Acidimicrobiia可以使用反向氧化TCA (roTCA) 循环,而不是卡尔文-本森-巴什姆循环,成为化学性自营. 这一发现凸显了这种微生物群体的潜在生态意义.
科学领域:
- 微生物学 微生物学
- 生物地质化学生物地质化学
- 进化生物学 进化生物学
背景情况:
- 酸性微生物是无处不在的微生物,通常通过卡尔文-本森-巴什姆 (CBB) 循环被认为是自营的.
- 在Acidimicrobiia中,化学和自的直接证据很少,这使得它们的碳固定途径不清楚.
研究的目的:
- 为了研究Acidimicrobiia的化学和自营养能力.
- 为了确定这些微生物使用的特定碳固定途径.
- 了解酸性微生物中碳固定机制的进化历史.
主要方法:
- 从盐湖丰富培养物中分离和描述了一种新型的化学物质自转基因,Salinilacustristhrix flava EGI L10123T.
- 基因组和转录组分析以确定代谢途径.
- 酶分析和稳定同位素探测以确认代谢活性.
主要成果:
- 菌株EGI L10123T属于一个新的Acidimicrobiia家族,表现出自营生长,但缺乏CBB循环基因.
- 有证据表明,逆氧化TCA (roTCA) 循环利用硫化物氧化作为主要的碳固定途径.
- 遗传学分析表明,roTCA基因在Acidimicrobiia中是古老的,而CBB基因是通过横向基因转移获得的.
结论:
- 通过roTCA循环的化学和自是一种可信的,潜在的古老的碳固定模式在Acidimicrobiia.
- 卡尔文-本森-巴什姆循环似乎是在一些血统中二次获得的.
- 这项研究扩大了我们对微生物碳循环和Acidimicrobiia生态作用的理解.
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