相关的2C型蛋白酸酶Pic3和Pic12负面调节番茄对Pseudomonas注射器的免疫力
Fan Xia1,2, Ning Zhang1,2, Renee E Smith1
1Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.
Plant physiology
|July 29, 2024
概括
两种2C型蛋白酸酶 (PP2Cs),Pic3和Pic12,可以负面调节番茄对Pseudomonas syringae pv.的免疫力. 番茄 番茄 番茄 番茄 番茄 这些基因的功能丧失突变增强了植物的抵抗力,这表明它们在调节防御通路中的作用.
科学领域:
- 植物生物学 植物生物学
- 分子植物病理学 分子植物病理学
- 植物免疫力 植物免疫力
背景情况:
- 2C型蛋白酸酶 (PP2Cs) 是植物中的一个大型基因家族,在植物免疫力中已知作用有限.
- 了解特定PP2Cs在植物防御反应中的作用,对于开发抗病作物至关重要.
研究的目的:
- 为了识别和描述参与番茄 (Solanum lycopersicum) 免疫的PP2C酸酶.
- 研究Pic3和Pic12在对细菌病原体Pseudomonas syringae pv.抗药性中的作用. 番茄 (Pst) 的使用情况.
主要方法:
- 在番茄中的11个PP2C编码基因中产生了功能丧失突变.
- 评估了突变系中的Pst耐药性以及Nicotiana benthamiana中的过度表达.
- 进行RNA测序来分析野生类型和突变植物的防御基因表达.
主要成果:
- 在Pic3和Pic12中功能丧失突变增强了番茄对Pst的抵抗力,对不同叶子年龄产生不同的影响.
- 过度表达Pic3/12抑制了Pst抵抗,这取决于酸酶活性和棕化动机.
- 组合突变 (RG-pic3 / RG-pic12) 显示出比单个突变更强的耐药性,这表明了附加效应.
- 在RG-pic3突变体中增强的耐药性与与防御相关基因的表达增加相关.
结论:
- 皮克3和皮克12作为番茄对Pst.免疫力的负调节剂.
- 它们在防御中的功能是附加的,并通过鞭毛素独立的途径运作.
- 这些PP2C代表了提高作物抗病能力的潜在目标.
相关概念视频
01:20The JAK-STAT Signaling Pathway
8.8K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.8K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
02:57piRNA - Piwi-interacting RNAs
6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:42Phosphoinositides and PIPs
8.5K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.5K
02:43Plant Cell Wall
56.5K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.5K