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双甲和双AF的联合暴露通过线粒体功能障碍诱导了人类卵巢颗粒细胞的亡

Mingquan Huang¹, Guofeng Xu², Mi Li³

¹Sichuan Treatment Center for Gynaecologic and Breast Diseases (Breast Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|July 29, 2024

概括

这项研究揭示了双A (BPA) 和双AF (BPAF) 的同时暴露会导致人类颗粒细胞中显著的线粒体功能障碍和压力,导致细胞亡. 这些发现强调了混合双暴露的细胞毒性.

科学领域:

内分泌学在内分泌学.
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

双A (BPA) 在塑料和树脂中广泛使用,已知具有生殖毒性.
关于BPA及其衍生物Bisphenol AF (BPAF) 的联合作用的数据有限.
颗粒状细胞对于女性的繁殖至关重要.

研究的目的:

调查BPA和BPAF同时暴露对人类颗粒细胞 (KGN细胞) 中线粒体功能的影响.
阐明二醇诱导的线粒体功能障碍和亡背后的机制.

主要方法:

在24小时内,KGN细胞单独和组合暴露于BPA (30,50微米) 和BPAF (3,5微米) 的不同度.
评估了线粒体质量,ATP生产,呼吸链功能,水平和活性氧物种 (ROS).
用抗氧化剂和化剂预处理细胞,以确定线粒体应激在亡中的作用.

主要成果:

暴露于高度的BPA和/或BPAF诱导显著的线粒体功能障碍,其特征是线粒体质量减少,ATP生产和呼吸链损伤.
联合BPA和BPAF暴露增加了线粒体水平和ROS产量,表明线粒体应激增加.
线粒体应激被确定为促进KGN细胞中亡的关键机制.

结论:

同时暴露于BPA和BPAF会在人体颗粒细胞中诱导显著的线粒体功能障碍和压力.
这种线粒体功能障碍有助于双醇诱导的亡,影响女性的生殖健康.
这项研究为混合双化合物暴露的细胞毒性提供了新的证据.

关键词:

双 A 是一种双.双 AF AF 是一种双.共同暴露共同暴露颗粒素细胞是颗粒素的细胞.线粒体功能障碍线粒体功能障碍氧化应激是一种氧化应激.

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