拉曼超光谱成像作为一种潜在的工具,用于快速和非破坏性地识别玉米核中的亚拉托克辛污染
Feifei Tao1, Haibo Yao2, Zuzana Hruska3
1Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, USA; USDA-ARS, Environmental Microbial and Food Safety Laboratory, Beltsville, MD 20705, USA.
Journal of food protection
|July 29, 2024
概括
拉曼超光谱成像有效检测到玉米核中的 aflatoxin污染,细菌侧分析显示更高的准确性. 这种方法为确保食品安全提供了一个有希望的方法.
科学领域:
- 农业科学 农业科学
- 食品安全 食品安全
- 分析化学 分析化学
背景情况:
- 毒素是真菌产生的有毒二次代谢物,对食品安全和人类健康构成重大威胁.
- 准确和快速检测玉米等农产品中的偏毒素污染,对于防止经济损失和保护消费者至关重要.
研究的目的:
- 评估拉曼高光谱成像 (RHI) 检测玉米核中亚亚黄毒素污染的疗效.
- 为了比较RHI的性能,使用不同的光谱区域 (内和胚芽) 和数据处理方法.
主要方法:
- 人工注射玉米核的亚弗拉托基因和非亚弗拉托基因真菌.
- 拉曼超光谱成像采集使用785nm激光.
- 主要组件分析-线性差异分析 (PCA-LDA) 建模使用各种光谱输入 (全光谱,预处理光谱,局部峰值).
主要成果:
- 细菌侧的光谱分析显示,与内精子侧的分析相比,其性能优越.
- 使用预先加工的胚胎侧光谱,对亚黄毒素检测的预测准确率高达86.7% (20ppb值) 和89.6% (100ppb值).
- 使用两个内核侧原始光谱的模型实现了最好的整体预测性能,平均准确率为81.8% (20ppb) 和84.5% (100ppb).
结论:
- 拉曼超光谱成像是一种可行的技术,用于检测玉米核中的偏毒素污染.
- 优化从胚胎端的光谱采集和适当的数据处理显著提高了检测准确度.
- 这种RHI方法为玉米行业的质量控制提供了一种快速,非破坏性的方法.
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