细菌纳米纤维素通过静态,静态间歇料批和旋转盘生物反应器基础的发酵路线使用经济的黑茶介质:一个比较账户
Chhavi Sharma1, Nishi K Bhardwaj2, Puneet Pathak3
1University Institute of Biotechnology, Chandigarh University, Mohali 140413, India; University Centre for Research and Development, Chandigarh University, Mohali, -140413, India.
International journal of biological macromolecules
|July 29, 2024
概括
使用黑茶和SCOBY优化了经济的细菌纳米纤维素 (BNC) 生产. 静态间歇养批量 (SIFB) 方法产生了最高的BNC,证明了具有成本效益的工业应用潜力.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 细菌纳米纤维素 (BNC) 是一种多功能生物材料,具有众多应用.
- 传统的BNC生产方法通常依赖于昂贵的介质和特定的细菌菌株.
- 开发具有成本效益和高效的BNC生产策略对于更广泛的工业采用至关重要.
研究的目的:
- 为了比较静态,静态间歇养批量 (SIFB) 和旋盘生物反应器 (RDB) 模式在BNC生产中的有效性.
- 评估使用经济的黑茶介质和细菌和酵母 (SCOBY) 的共生联盟生产的BNC的物理化学特性.
- 确定最佳的生产方法,以最大限度地提高BNC产量和质量.
主要方法:
- 在静态,SIFB和RDB生物反应器模式中,使用黑茶和SCOBY生产了BNC.
- 对BNC的物理化学表征包括FE-SEM,ATR-FTIR,XRD和TGA.
- 每种模式的BNC产量,糖消耗,转换产量和剩余糖都被量化.
主要成果:
- 产生的BNC表现出多孔形态,主要是Iα形式,良好的结晶性 (68-79.4%) 和热稳定性.
- 在所有模式中观察到高水率 (86-93%) 和水分含量 (85-93%).
- SIFB方法实现了最高的BNC收益率 (29.4 gL-1),显著超过静态模式 (13.6 gL-1).
- 在RDB模式下,可忽略不计的BNC (1.0gL-1),并且发现SCOBY与黑茶不适合此模式.
结论:
- SIFB方法提供了一种具有成本效益和高收益的方法,用于使用经济媒介生产细菌纳米纤维素.
- 生产的BNC具有理想的物理化学特性,适用于各种技术工业应用.
- 需要进一步调查,以优化在RDB模式下使用SCOBY和黑茶的BNC生产.
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