在早期发病的严重预先怀孕症中,等级性 lncRNA 调控网络
Haihua Liu1,2,3,4,5,6, Zhijian Wang1,2,3,4, Yanjun Li1,2,3,4,5,6
1Center for Genetics and Developmental Systems Biology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
BMC biology
|July 29, 2024
概括
长非编码RNAs (lncRNAs) 在早期发病的严重孕前 (EOSPE) 中发挥作用. 研究人员确定了一个涉及lncRNAs,转录因子和基因的调节网络,揭示FLNB-AS1是EOSPE病变发生的关键参与者.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生殖生物学 生殖生物学
背景情况:
- 孕前 (PE) 是一种与胎盘异常相关的妊娠并发症.
- 几种长非编码RNA (lncRNAs) 涉及PE,但它们在疾病进展中的确切作用尚不清楚.
研究的目的:
- 阐明 lncRNAs 在早期发病的重症预孕 (EOSPE) 病理进展中的作用.
- 在EOSPE胎盘中构建和分析 lncRNAs,转录因子 (TFs) 和差异表达基因 (DEGs) 的等级调节网络.
主要方法:
- 来自EOSPE患者和正常受试者的胎盘组织的转录组测序.
- 构建一个 lncRNA-TF-DEG 层次性的监管网络.
- 使用三重结构验证 lncRNA-促进体相互作用.
- 对DElncRNAs与血压等临床参数的相关性分析.
主要成果:
- 在EOSPE胎盘中识别差异表达基因 (DEGs) 和lncRNAs (DElncRNAs).
- 构建一个具有1851 lncRNA-TF和6901 TF促进体相互作用的网络.
- DElncRNAs的聚类揭示了FLNB-AS1,它准了869个DEG,与血压相关.
- FLNB-AS1被证明可以结合JUNB,并调节 trofhoblast细胞中的HIF-1信号通路.
结论:
- lncRNAs的差异表达扰乱了等级调节网络,导致了EOSPE中的基因失调.
- 这项研究为研究EOSPE背后的分子机制提供了一种新的策略和资源.
更多相关视频
07:36Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
Published on: November 20, 2015
11.3K
05:30Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
391
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:33Hormonal Regulation
33.0K
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
33.0K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:23Regulation of Expression at Multiple Steps
880
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
880
01:37Neural Regulation
39.2K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
39.2K
01:31Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K