相关实验视频

Updated: Jun 18, 2025

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Published on: January 6, 2014

在 Pseudomonas aeruginosa 感染期间,cUMP会引起细胞间隙的形成

Althea deWeever^1,2, Sunita S Paudel^1,2, Chun Zhou^1,2

¹Department of Physiology and Cell Biology, University of South Alabama, Mobile, Alabama, United States.

American journal of physiology. Lung cellular and molecular physiology

|July 30, 2024

概括

伪omonas aeruginosa感染通过产生循环UMP (cUMP),一种以前与肺损伤无关的分子,引起肺. 这项研究证明了cUMP.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
肺部医学肺部医学

背景情况:

Pseudomonas aeruginosa 使用 3 型分泌系统 (T3SS) 将毒素注入宿主细胞.
细菌酶ExoY产生像cAMP,cGMP和cUMP这样的循环核酸.
虽然已知cAMP和cGMP的作用,但cUMP在P. aeruginosa感染中的功能尚不清楚.

研究的目的:

调查周期性UMP (cUMP) 在P. aeruginosa感染期间内皮细胞屏障破坏中的作用.

主要方法:

使用一种膜透的cUMP模拟器 (cUMP-AM).
感染培养的肺微血管内皮细胞 (PMVECs) 具有催化不活跃的 ExoY.
在隔离的透气肺中评估了内皮间隙形成和过系数.

主要成果:

外源性cUMP诱导PMVEC中的内皮间隙形成.
cUMP增加了隔离 perfused 肺中的过系数.
这些效应甚至发生在具有催化不活性的EXOY时,表明cUMP的直接作用.

结论:

循环UMP (cUMP) 直接有助于增加内皮膜的通透性.
在P. aeruginosa肺部感染期间,cUMP在引发肺方面发挥着重要作用.
了解cUMP的功能为治疗P. aeruginosa引起的肺损伤开辟了新的途径.

关键词:

循环核酸单酸盐的核酸.外酵素 Y 是一种外酵素.透性透性的肺炎是一种肺炎.第三类分泌系统.

更多相关视频

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Published on: August 14, 2014

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

相关概念视频

Adherens Junctions

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic

Gap Junctions

Gap Junctions

The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Inflammation

Inflammation