关于 Campylobacter concisus 的可生存但不可培养状态的表征
Syeda Umme Habiba Wahid1, Bronwyn E Campbell1, Robert J Moore1
1School of Science, RMIT University, Bundoora, Victoria, Australia.
Royal Society open science
|July 30, 2024
概括
在环境压力下,Campylobacter concisus细菌可以进入可生存但不可培养 (VBNC) 状态. 这种以形态变化为特征的生存机制,对于细菌的持久性和传播至关重要.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 环境科学 环境科学
背景情况:
- 坎皮洛巴克特 (Campylobacter concisus) 是一种机会性病原体,涉及到各种人类疾病.
- 了解细菌生存机制,如可生存但不可培养 (VBNC) 状态,对于公共卫生至关重要.
研究的目的:
- 调查Campylobacter concisus中VBNC状态的诱导和特征.
- 确定不同C. concisus菌株VBNC状态进入的时间框架.
主要方法:
- 诱导VBNC状态,通过在酸盐缓冲盐水中保持C.concisus在4°C下三周.
- 使用一氧化与定量实时PCR (PMAxx-qPCR) 结合使用可活细胞的量化,针对DNA旋转酶子单元B基因.
- 对VBNC细胞进行形态分析.
主要成果:
- C. concisus ATCC 51562 在15天内进入了VBNC状态,而ATCC 51561 在9天内进入了VBNC状态.
- PMAxx-qPCR证实了高比例的细胞在VBNC状态,可活细胞数量接近10^7 CFU/ml.
- VBNC细胞表现出形形态和缩小的大小.
结论:
- 环境压力会在大多数C. concisus细胞中诱导VBNC状态.
- VBNC状态是C. concisus的重要生存策略,可能有助于其在环境中的持久性和传播.
- 在环境和临床监测策略中应考虑VBNC C. concisus的存在.
更多相关视频
08:23Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
11.5K
05:34Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
1.8K
相关概念视频
Bacterial Flora of the Large Intestine
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Stringent Response in E. coli
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Bacterial Gastroenteritis
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
