直接的三维质谱成像与激光消光 遥控大气压力 光电化/化学电离
Tomasz Ruman1, Sumi Krupa2, Joanna Nizioł1
1Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of Technology, 6 Powstan ́ców Warszawy Ave., Rzeszów 35-959. Poland.
Analytical chemistry
|July 30, 2024
概括
研究人员开发了一种新的激光切除技术,用于直接3D质谱成像组织中的代谢物. 这种方法避免了样本切片,并揭示了癌症与正常组织和植物结构中的代谢物差异.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 代谢学 代谢学 代谢学
背景情况:
- 生物组织中代谢物的直接3D质谱成像 (MSI) 是具有挑战性的.
- 现有的方法往往需要复杂的样本准备,如薄切割.
研究的目的:
- 开发和验证一种新的平台,用于直接3DMSI的代谢物,而无需样本修改.
- 展示新系统分析人类和植物组织的能力.
主要方法:
- 开发一个激光除远程大气压光电离/化学电离 (LARAPPI/CI) 平台.
- 配合LARAPPI/CI与超高分辨率的四极飞行时间 (QToF) 质谱仪.
- 使用衍射光学元件进行受控的组织层去除 (voxel堆叠).
- 采用组合的剂辅助大气压光电离 (DA-APPI) 和大气压化学电离 (APCI) 离子源.
主要成果:
- 在凝,红卜,菜和人类脏组织 (癌症和正常) 中成功地直接3DMSI代谢物.
- 高分辨率的二维和三维离子图像清楚地区分了代谢物丰度差异.
- 证明了可视化癌症和非癌症脏区域之间的代谢变异的能力.
- 展示了该系统在区分各种植物组织内的代谢物概况方面的实用性.
结论:
- LARAPPI/CI MSI平台能够直接,无标签地对完整组织中的代谢物进行3D可视化.
- 开发的voxel堆叠方法有效地允许在不进行分割的情况下进行深度分析.
- 这项技术为临床和植物学研究中的比较代谢学提供了强大的工具.
- 联合DA-APPI和APCI离子源代表了MSI应用的新进展.
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