在性结肠炎的老鼠模型中,用牙纸干细胞和硫沙拉的组合干预
Riham M Aly1,2, Rehab S Abohashem3,4, Hanaa H Ahmed3,4
1Basic Dental Science Department, Oral & Dental Research Institute, National Research Centre, 33 El Bohouth St, Dokki, Giza, Egypt. rm.el-sayed@nrc.sci.eg.
Inflammopharmacology
|July 30, 2024
概括
将牙纤维干细胞 (DPSC) 与硫沙结合使用,在治疗大肠发炎的老鼠中显示出协同效应. 这种组合有效降低了炎症和氧化应激,为炎症性肠病提供了有前途的治疗策略.
科学领域:
- 再生医学是一种再生医学.
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 性结肠炎 (UC) 是一种炎症性肠病 (IBD),其特点是结肠和直肠的慢性炎症.
- 目前对UC的治疗旨在减少炎症,但最终治愈仍然难以捉摸.
- 研究新的治疗组合对于管理UC症状和改善患者治疗结果至关重要.
研究的目的:
- 为了评估将牙纤维干细胞 (DPSC) 与酸诱导的性结肠炎老鼠模型中的硫沙拉结合的治疗疗效.
- 评估这种组合治疗对抑制炎症性细胞因子和调节氧化应激 in vivo 的影响.
- 确定DPSCs和硫素在改善UC中的协同作用.
主要方法:
- 性结肠炎在老鼠中被诱导使用3%的乙酸通过直肠给药.
- 通过测量结肠体重/长度比率,瘤标志物和进行组织病理学分析来评估治疗效果.
- 免疫组织化学和ELISA用于评估NF-κB-P65,IL-1β,TNF-α和TLR-4的表达,以及氧化应激和抗氧化剂指数. qRT-PCR分析了TLR-4,NF-κB-P65和MYD88.8的基因表达.
主要成果:
- 联合使用DPSCs和硫素显著改善了宏观和显微的炎症迹象,恢复了组织结构.
- 组合疗法显著降低了活性氧物种 (ROS) 水平,并增加了山丁氧化酶 (XOD) 活性.
- 免疫组织化学和基因表达分析显示,DPSCs和硫素减弱了NF-κB-p65,IL-1β,MyD88和TLR4的表达.
结论:
- 在性结肠炎的实验模型中,用DPSC和硫沙拉联合治疗表明了协同治疗效应.
- 这种组合有效地抑制了炎症反应,并调节了氧化应激标志物.
- 这些发现表明,联合DPSC和硫沙治疗在治疗性结肠炎方面具有有希望的潜力.
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