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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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相关实验视频

Updated: May 8, 2026

$Synthesis and Microdiffraction at Extreme Pressures and Temperatures$

Synthesis and Microdiffraction at Extreme Pressures and Temperatures

Published on: October 7, 2013

使用衍射有限存储环进行时间分辨率成像的新机会.

Zisheng Yao¹, Julia Rogalinski¹, Eleni Myrto Asimakopoulou¹

¹Synchrotron Radiation Research and NanoLund, Department of Physics, Lund University, Lund, Sweden.

Journal of synchrotron radiation

|July 30, 2024

概括

新的X射线显微镜技术利用衍射有限存储环 (DLSR) 提高流量密度. 这可以实现高速,高分辨率的成像,在ForMAX光线线上达到每秒2000.

关键词:

在ForMAX光线线上马克思四 MAX IV 马克思四衍射有限储存环的储存环.千兆赫兹成像成像系统时间分辨率显微镜.

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Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

相关实验视频

Last Updated: May 8, 2026

$Synthesis and Microdiffraction at Extreme Pressures and Temperatures$

Synthesis and Microdiffraction at Extreme Pressures and Temperatures

Published on: October 7, 2013

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

科学领域:

在X射线显微镜中使用X射线显微镜.
材料科学是一种材料科学.
光子科学是一种科学.

背景情况:

分散有限储存环 (DLSR) 的亮度明显高于前几代.
来自DLSR的光子流密度增加使得先进的X射线成像技术成为可能.
时间分辨率的X射线显微镜需要高流量和分辨率的动态过程研究.

研究的目的:

在MAX IV储存环上对ForMAX光线的时间分辨率显微镜能力进行基准测试.
评估DLSR在高空间时空分辨率X射线成像方面的潜力.
为了证明可以通过增强流量密度实现的先进成像速率.

主要方法:

在MAX IV储存环上使用ForMAX光线.
使用X射线源的单个波.
使用探测器系统的全部动态范围对成像性能进行基准测试.

主要成果:

实现了微米分辨率的时间分辨率成像.
已证明的获取率为每秒2000.
达到1.1 MHz的2D采集率,并增强了流量密度.

结论:

DLSR为先进的X射线显微镜提供了前所未有的流量密度.
ForMAX光线线展示了最先进的时间分辨率成像能力.
高速,高分辨率的X射线成像对于研究动态现象是可行的.