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Updated: Jun 18, 2025

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

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基于酶的选择性样本处理 - - 无歧视和检测特定站点DNA5 - - 氧甲基细胞因子

Jiahui Zhao1, Jingli Yan1, Jing Li1

  • 1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province (Project Number: 22567620H), State Key Laboratory of New Pharmaceutical Preparations and Excipients, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.

Analytical chemistry
|July 30, 2024
PubMed
概括

这项研究引入了一种选择性酶介导机制 (SLim),用于在DNA中直接检测5-基甲基细胞素 (5hmC). SLim可以将5hmC与没有复杂样本准备的其他形式区分开来,从而能够进行敏感的量化.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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