从传统酸面粉中分离出来的乳酸细菌的分子特征和多功能评估
Yeşim Aktepe1, Furkan Aydın2, Tuğba Bozoğlu3
1Department of Food Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, 14030, Bolu, Türkiye.
International journal of food microbiology
|July 30, 2024
概括
这项研究确定了来自酵母面的有希望的乳酸细菌 (LAB) 菌株,用于发酵食品中的功能起始剂. 几种菌株显示出出色的技术和益生菌潜力,与Lactobacillus rhamnosus G.G.相当.
科学领域:
- 食品微生物学 食品微生物学
- 应用微生物学应用微生物学
- 细菌学 细菌学是一门学科.
背景情况:
- 酵母面的发酵依赖于乳酸细菌 (LAB) 进行风味和保存.
- 识别具有功能性和益生菌性质的新型LAB菌株对于食品应用至关重要.
研究的目的:
- 从酸面粉中分离和鉴定本土乳酸细菌 (LAB).
- 评估选择的LAB菌株的技术和益生菌潜力,以作为发酵食品中的功能启动剂.
主要方法:
- 350个LAB菌株的隔离和随机放大多态DNA (RAPD) 分析.
- 使用16S rRNA基因测序进行物种识别.
- 技术特征 (酸度,EPS,发酵,耐压力) 和益生菌评价 (pH/胆存活率,聚合,抗氧化剂/抗菌活性).
主要成果:
- 确定的LAB物种是:康帕尼拉科巴西勒斯克鲁斯托勒姆,莱维拉科巴西勒斯布雷维斯,拉蒂拉科巴西勒斯曲线,康帕尼拉科巴西勒斯偏食和拉蒂拉科巴西勒斯植物.
- 选择了16种技术相关的菌株 (2 Lp. 植物体,14 利未记. 布雷维斯),有些人表现出对四环素的耐药性.
- 确定了3种菌株 (Lp. 脚部 L7.8, 列王记. 布雷维斯 (L55.1,L62.2) 的益生菌潜力相当于Lactobacillus rhamnosus GG. 的益生菌潜力.
结论:
- 选择的LAB菌株具有显著的技术和益生菌特征.
- 乳酸菌植物菌L7.8和乳酸菌L55.1和乳酸菌L62.2是和谷物发酵产品中功能性起始培养的有希望的候选人.
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