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Updated: Jun 18, 2025

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

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在生菜 (Lactuca sativa L.) 中组织特异性基因和异型的高通量单分子长读RNA测序分析

Zhuo-Xing Shi1,2, Lei Xiang1, Hai-Ming Zhao1

  • 1Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.

Communications biology
|July 30, 2024
PubMed
概括

我们开发了HIT-ISOseq,这是一种高通量方法,用于在生菜中长时间读取RNA测序. 这种技术改善了基因注释,并揭示了组织特异性的基因表达,推动了蔬菜的基因组研究.

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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