相关实验视频

Updated: Jun 18, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

与不稳定的RNA相关的增强剂在植物中很罕见

Bayley R McDonald¹, Colette L Picard², Ian M Brabb¹

¹School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.

|July 30, 2024

概括

不稳定的转录,在植物中很少见,与脊椎动物不同. 来自远端元素的稳定转录作为促进剂,影响增强剂活性,并突出突出了真核生物中多样化的 cis 调节机制.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

不稳定的转录是已知的脊椎动物增强剂标记物,参与细胞过程和疾病.
它们在植物中的流行和功能在很大程度上仍未得到研究.
了解植物的 cis-regulatory 元素对于作物改良和基本生物学至关重要.

研究的目的:

综合调查植物中不稳定转录的流行率和特征.
确定不同转录类型在植物增强剂活性中的作用.
探索植物基因组中cis调节元素的多样性.

主要方法:

覆盖小 (cs) RNA 测序以捕获各种植物物种的新生转录.
集成自转录活性调节区域 (STARR) 测序数据以评估增强器功能.
对转录稳定性,起源和调节元素活动的比较分析.

主要成果:

与脊椎动物相比,不稳定的转录在植物中很少见,并且通常来自促进者.
植物中的远端元素经常启动特定于组织的稳定转录,这表明了新的促进器作用.
发起稳定转录的区域,而不是不稳定的转录,在STARR-seq试验中表现出更强的增强剂活性.

结论:

在不稳定的转录方面,植物的 cis-regulatory 景观与脊椎动物有很大差异.
来自包括远端元素在内的各种基因组位置的稳定转录是增强剂活性的关键驱动因素.
促进剂和增强剂之间的区别是模糊的,具有不同的真核细胞 cis-regulatory 元素.

更多相关视频

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

相关概念视频

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...