乳球菌ICE-ome编码了一系列可交换的特征,具有潜在的工业相关性
Simon van der Els1,2,3, Jos Boekhorst1, Peter A Bron2,3
1Host-Microbe Interactomics Group, Department of Animal Sciences, Wageningen University & Research, De Elst 1, Wageningen, 6708 WD, The Netherlands.
BMC genomics
|July 30, 2024
概括
研究人员在乳球球菌基因组中确定了25个整合性结合元件 (ICE),揭示了各种货物基因. 这些移动遗传元素 (MGE) 提供了通过菌体抵抗和应激适应等功能来改善乳制品初始培养的潜力.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 基因组学就是基因组学.
背景情况:
- 乳球菌种对于乳制品发酵至关重要,初始培养创新侧重于增强功能.
- 移动遗传元素 (MGE),特别是整合性结合元素 (ICE),显著影响细菌生物多样性和基因获取.
- 了解ICE的边界和货物基因对于利用其在工业应用中的潜力至关重要.
研究的目的:
- 准确地界定乳球菌基因组中的整合结合元件 (ICE).
- 为了识别和描述这些ICE所携带的货物基因.
- 评估由乳球菌ICEs编码的功能的工业相关性.
主要方法:
- 使用插入部位侧边单拷贝核心基因组基因,在低邻的乳球菌基因组中划分25个ICE.
- 在CRISPR-Cas9中介的ICE-curing之后进行了重新排序,以便在高结合基因组中准确划分.
- 在26个受界的乳球球菌ICE中对货物基因库进行比较分析.
主要成果:
- 在乳球菌基因组中成功划分了25个ICE,识别了不同的ICE整合酶.
- 证明了CRISPR-Cas9介导的ICE固化作为ICE划界和货物定义的有效方法.
- 在乳球菌ICE的货物基因谱中发现了显著的可塑性,具有显著的功能,包括限制修饰,流产性感染和应激适应.
结论:
- 使用基因组标记物和CRISPR-Cas9.9等先进技术,可以实现ICE的准确划界.
- 乳球球菌ICE含有各种各样的货物基因,对乳制品初始培养的发展具有重要的工业潜力.
- 这些已识别的基因为提高发酵乳制品中初始培养强度,菌体耐药性和耐压力提供了机会.
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