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转录组和加权基因共同表达网络的分析揭示了关键的基因和途径,涉及到早熟水果在Citrus sinensis中成熟

Jianmei Chen¹, Lihong Xie¹, Yi Lin¹

¹National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Jiangxi, Ganzhou, 341000, P R China.

|July 30, 2024

概括

早期类水果在夏季成熟.

科学领域:

植物科学植物科学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

果实成熟是类繁殖的一个关键特征.
开发早期成熟的品种可以优化成熟期.
了解监管网络对于类品种育种计划至关重要.

研究的目的:

在Citrus sinensis中调查果实成熟的调节网络.
让我们比较一下早期成熟的突变种"Gannanzao"和"Newhall"的腹色.
确定控制果实早期成熟的关键基因和途径.

主要方法:

对"Gannanzao"和"Newhall"腹的生理分析.
RNA测序用于识别差异表达基因 (DEG).
功能性丰富分析和加权基因共同表达网络分析 (WGCNA).

主要成果:

早期成熟与酸 (ABA),斯酸 (JA) 和糖有关.
已发现1430个DEG,富含激素信号和糖代谢通路.
两个乙烯反应因子13 (ERF13) 基因 (Cs_ont_5g000690和Cs_ont_5g000700) 被确定为关键调节者.

结论:

鉴定了与果成熟相关的ABA/JA代谢,信号转导和糖代谢中的基因.
两个ERF13基因是Citrus sinensis中早期果实成熟的关键调节者.
研究结果提供了关于类品种早期成熟的遗传基础的见解.

关键词:

(Citrus sinensis) 是一种类植物.果实成熟的时间转录组转录组就是一个转录组.在WGCNA中,WGCNA是WGCNA.

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