在冬季小麦中表观基因组识别本土化控制因素
Yanhong Liu1,2, Pan Liu1, Lifeng Gao1
1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Genome biology
|July 31, 2024
概括
冬季小麦的本地化包括调节开花的表观遗传变化. 这项研究确定了关键的调节元素和TaSPL7/15基因.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 小麦遗传学 小麦遗传学
背景情况:
- 冬季小麦需要长时间的寒冷 (冬化) 才能开花.
- 绿化触发了开花信号,但抑制了种子的生长.
- 关键的监管要素和机制在很大程度上是未知的.
研究的目的:
- 为了全面描述冬季小麦本土化过程中的表观基因组和转录基因组变化.
- 为了确定新的cis-regulatory元素和控制本地化的网络.
- 阐明与衰老相关的基因在本地化途径中的作用.
主要方法:
- 广泛的表观基因组和转录基因组在叶子,尾芽和射门顶部组织上进行分析.
- 对表观遗传修饰的分析,如H3K27me3.3.
- 整合多主题数据来构建监管网络.
主要成果:
- 在本地化过程中,表观遗传修饰驱动组织特异性和亚基因组分离基因表达.
- H3K27me3主要调节源于本地化的基因.
- 确定了10600个假定的本地化调节元素,包括VRN3.3附近的远程ACR.
- 发现TaSPL7/15与VRN1促进剂和VRN3调节元件相互作用,影响开花.
结论:
- 提供了对小麦本地化表观基因组学的关键见解.
- 确定了用于开发小麦的监管要素,并提供了量身定制的本土化反应.
- 建立了一个涉及TaSPL7/15的本地化转录网络,从衰老路径.
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